Human Fucosyltransferase 11/FUT11 Antibody

Catalog #: AF5964 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5964
AF5964-SP

Detection of Human Fucosyltransferase 11/FUT11 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Fucosyltransferase 11/FUT11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FUT11 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FUT3, rhFUT5, and rhFUT8 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 11/FUT11
Gly25-Leu492
Accession # AAH36037

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 11/FUT11 (Catalog # 5964-GT), see our available Western blot detection antibodies

Immunocytochemistry

5-15 µg/mL

Immersion fixed NTera-2 human testicular embryonic carcinoma cells, PC‑3 human prostate cancer cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Fucosyltransferase 11/FUT11 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Fucosyltransferase 11/FUT11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5964) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Fucosyltransferase 11/FUT11 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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Fucosyltransferase 11/FUT11 in NTera‑2 Human Cell Line. Fucosyltransferase 11/FUT11 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Human Fucosyltransferase 11/FUT11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5964) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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Detection of Fucosyltransferase 11/FUT11 in PC‑3 cells Fucosyltransferase 11/FUT11 was detected in immersion fixed PC‑3 cells (Positive) using Sheep Anti-Human Fucosyltransferase 11/FUT11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5964) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fucosyltransferase 11/FUT11

Fucose is frequently found at terminal sites on various glycans and is essential for the generation of many sugar epitopes. Well-known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in leukocyte homing and extravasation process, thus are essential for lymphocyte maturation and natural defense functions (1). O-fucosylation on Notch receptor is found to be essential for its signaling function (2). So far, 11 carbohydrate specific fucosyltransferases are found in humans (3). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood group antigen synthesis (4). FUT3, FUT4, FUT5, FUT6, FUT7, and FUT9 are alpha 1-3/4 fucosyltransferases and are responsible for Lewis antigen generation (5). FUT10 and FUT11 are newly cloned alpha 1-3 fucosyltransferases that are distinct from the alpha 1-3/4 fucosyltransferase subfamily and are able to introduce fucose to the innermost core GlcNAc of the N-glycan on conalbumin glycopeptides and biantennary N-glycan acceptors but not onto short lactosaminyl acceptor substrates (6). Predicted as a type II transmembrane protein and a Golgi resident enzyme, the exact function of this enzyme needs to be further characterized.

References

Weston, B.W. et al. (1992) J. Biol.Chem. 267:4152.
Stahl, M. et al. (2008) J. Biol.Chem. 283:13638.
Becker, D.J. et al. (2003) Glycobiology 13:41R.
Kelly, R.J. et al. (1995) J. Biol.Chem. 270:4640.
Weston, B.W. et al. (1992) J. Biol.Chem. 267:24575.
Mollicone, R. et al. (2009) J. Biol. Chem. 284:4723.

Entrez Gene IDs

170384 (Human); 73068 (Mouse); 286971 (Rat)

Alternate Names

alpha-(1,3)-fucosyltransferase 11; EC 2.4.1; EC 2.4.1.-; fucosyltransferase 11 (alpha (1,3) fucosyltransferase); Fucosyltransferase 11; Fucosyltransferase XI; Fuc-TXI; fucT-XI; FUT11; Galactoside 3-L-fucosyltransferase 11; MGC119338; MGC119339; MGC33202