Home / Fucosyltransferase 7/FUT7 / Human Fucosyltransferase 7/FUT7 Antibody

Human Fucosyltransferase 7/FUT7 Antibody

Catalog #: MAB64091
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB64091
MAB64091-SP
Detection of Human Fucosyltransferase 7/FUT7 by Western Blot.
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Product Details
Citations (3)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Fucosyltransferase 7/FUT7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fucosyltransferase 7/FUT7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Fucosyltransferase 5 is observed.
Source
Monoclonal Mouse IgG2A Clone # 795116
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 7/FUT7
Ala36-Ala342
Accession # Q11130
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Fucosyltransferase 7/FUT7 antibody by Western Blot. View Larger

Detection of Human Fucosyltransferase 7/FUT7 by Western Blot. Western blot shows lysates of HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Fucosyltransferase 7/ FUT7 Monoclonal Antibody (Catalog # MAB64091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fucosyltransferase 7/FUT7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Fucosyltransferase 7/FUT7 antibody in HL-60 Human Cell Line by Immunocytochemistry (ICC). View Larger

Fucosyltransferase 7/FUT7 in HL‑60 Human Cell Line. Fucosyltransferase 7/FUT7 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Fucosyltransferase 7/FUT7 Monoclonal Antibody (Catalog # MAB64091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fucosyltransferase 7/FUT7

N-glycans, O-glycans and glycolipids are frequently fucosylated at terminal sites. Therefore, fucose is often part of a sugar epitope with important biological function. Well known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in the leukocyte homing and extravasation process and thus are important for lymphocyte maturation and natural defense functions. Fucose-containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned (2). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood-group antigen synthesis. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans (3). FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha 1-3 or alpha 1-4 fucosyltransferases and are responsible for Lewis antigen generation. FUT7 plays an exclusive role for the biosynthesis of sialyl Lewis X epitope (NeuAc alpha 2,3Gal beta 1,4[Fuc alpha 1,3]GlcNAc) that serves as a ligand in the E-selectin and P-selectin mediated adhesion of leukocytes to activated endothelium or platelets (4, 5, 6). Expression of FUT7 is increased by hypoxia in human colon cancer cell lines (7). Within aa 36-342, human FUT7 shares 84% aa sequence identity with mouse and rat FUT7.

References
  1. Jafar-Nejad, H. et al. (2010) Glycobiology 20:931.
  2. Becker, D.J. et al. (2003) Glycobiology 13:41R.
  3. Lee, S.H. et al. (2006) J. Biochem. 139:391.
  4. Blander, J. M. et al. (1999) J. Immunol. 163:3746.
  5. Natsuka, S. et al. (1994) J. Biol. Chem. 269:16789.
  6. Sasaki, K. et al. (1994) J. Biol. Chem. 269:14730.
  7. Koike, T. et al. (2004) P.N.A.S. 101:8132.
Entrez Gene IDs
2529 (Human); 14347 (Mouse); 296564 (Rat)
Alternate Names
alpha-(1,3)-fucosyltransferase; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; FTVII; fucosyltransferase 7 (alpha (1,3) fucosyltransferase); Fucosyltransferase 7; Fucosyltransferase VII; Fuc-TVII; FucT-VII; FUT7; Galactoside 3-L-fucosyltransferase; Selectin ligand synthase; selectin-ligand synthase

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Citations for Human Fucosyltransferase 7/FUT7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1 alpha
    Authors: Xiaodan Wang, Suibing Miao, Linqi Lu, Jingchuan Yuan, Shuhong Pan, Xiaohua Wu
    International Journal of Molecular Medicine
  2. Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy
    Authors: F Colomb, LB Giron, L Kuri-Cerva, OS Adeniji, T Ma, H Dweep, E Battivelli, E Verdin, CS Palmer, H Tateno, AV Kossenkov, NR Roan, MR Betts, M Abdel-Mohs
    Cell Rep, 2020-08-04;32(5):107991.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. a-1,3-Fucosyltransferase-VII siRNA inhibits the expression of SLex and hepatocarcinoma cell proliferation.
    Authors: Li D, Sun H, Bai G et al.
    Int. J. Mol. Med.

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