Human FYB/ADAP/SLAP130 Antibody

Catalog #: MAB3857 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3857
MAB3857-SP

Detection of Human FYB/ADAP/SLAP130 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FYB/ADAP/SLAP130 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FYB/ADAP/SLAP130 in Western blots.

Source

Monoclonal Mouse IgG₃ Clone # 460114

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FYB/ADAP/SLAP130
Ala2-Gly274
Accession # O15117

Formulation

Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FYB/ADAP/SLAP130 by Western Blot. Western blot shows nuclear extracts of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Human FYB/ADAP/SLAP130 Monoclonal Antibody (Catalog # MAB3857) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FYB/ADAP/SLAP130 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FYB/ADAP/SLAP130

Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130 kDa (SLAP130), also known as Fyb-120 and ADAP, is an 86 kDa, 783 amino acid, cytoplasmic protein that runs anomalously as a 120‑130 kDa protein in SDS-PAGE. Human SLAP130 is a hematopoietic-specific adaptor that associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76 kDa (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known alternatively spliced SLAP130 isoform which contains an additional 46 aa. Over the region used as immunogen, SLAP130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.

Long Name

FYN Binding Protein (FYB-120/130)

Entrez Gene IDs

2533 (Human); 23880 (Mouse); 499537 (Rat)

Alternate Names

ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130SLAP-130FYN-binding protein (FYB-120/130); FYN binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP130; SLP-76-associated phosphoprotein