Human Gab2 Antibody

Catalog #: AF4877 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4877
AF4877-SP

Detection of Human Gab2 by Western Blot.

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Human Gab2 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Gab2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Gab2
Gln82-Phe292
Accession # Q9UQC2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Gab2 by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, U937 human histiocytic lymphoma cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human Gab2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4877) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Gab2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAB2

Gab2 (GRB2-associated binding protein 2) is a 95 kDa member of the Gab family of proteins. It is largely expressed in T and B cells, and serves as an adaptor molecule for the transmission of signals from cytokine and growth factor receptors. Bcr-Abl signals through Gab2, and Gab2 activates ERK. Human Gab2 is 676 amino acids (aa) in length, and contains one pleckstrin homology domain (aa 6‑117) and one methyl binding domain sequence (aa 454‑525). Multiple phosphorylation sites exist, with S159 being an important regulatory site. One alternate start site is reported at M39. Over aa 82‑292, human Gab2 is 93% and 98% aa identical to mouse and canine Gab2, respectively.

Long Name

GRB2-associated Binding Protein 2

Entrez Gene IDs

9846 (Human); 14389 (Mouse); 84477 (Rat)

Alternate Names

GAB2; GRB2-associated binding protein 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571GRB2-associated binder 2; p100; pp100