Human GAB3 Antibody

Catalog #: MAB7127 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7127
MAB7127-SP

Detection of Human GAB3 by Western Blot.

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Human GAB3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GAB-3 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 720306

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GAB3
Leu121-Asn263
Accession # Q8WWW8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GAB3 by Western Blot. Western blot shows lysates of human spleen tissue and human thymus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GAB3 Monoclonal Antibody (Catalog # MAB7127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAB3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAB3

GAB3 (GRB2-associated-binding protein 3) is a ~75-80 kDa member of the Gab family of scaffolding proteins which show overlapping expression in hematopoietic cells. All contain an N-terminal pleckstrin homology domain and multiple tyrosine phosphorylation and SH2 and SH3 domain binding sites. M-CSF stimulation of myeloid precursors through M-CSF R co-induces GAB3 and the associated adaptor protein GRAP2, and causes macrophage differentiation associated with tyrosine phosphorylation of GAB3. Over amino acids (aa) 121-263, human GAB3 shares 73% aa identity with mouse GAB3. A potential isoform of 548 aa lacks aa 509-548, while another of 488 aa lacks aa 173-356 and diverges at aa 475. A third potential isoform of 206 aa has an alternate start site at aa 380.

Long Name

GRB2-associated Binding Protein 3

Entrez Gene IDs

139716 (Human); 210710 (Mouse)

Alternate Names

DOS/Gab family member 3; Gab3 scaffolding protein; GAB3; GRB2-associated binder 3; GRB2-associated binding protein 3; GRB2-associated-binding protein 3; Growth factor receptor bound protein 2-associated protein 3