Human GAD1/GAD67 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2086
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Human GAD1/GAD67 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GAD1/GAD67 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human GAD2/GAD65 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GAD1/GAD67

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAD1/GAD67

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter gamma -amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

Long Name
Glutamate Decarboxylase 1 (Brain, 67kDa)
Entrez Gene IDs
2571 (Human)
Alternate Names
67kD); EC 4.1.1; FLJ45882; GAD1; GAD25; GAD67; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform

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