Human Galanin R1/GALR1 Antibody

Catalog #: MAB10353 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10353-100
MAB10353-SP

Detection of Galanin R1/GALR1 in HEK293 Human Cell Line Transfected with Human Galanin R1/GALR1 and eGFP by Flow Cytometry.

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Human Galanin R1/GALR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Galanin R1/GALR1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 613207

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Galanin R1/GALR1
Accession # P47211

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Galanin R1/GALR1 in HEK293 Human Cell Line Transfected with Human Galanin R1/GALR1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Galanin R1/GALR1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Galanin R1/GALR1 Monoclonal Antibody (Catalog # MAB10353) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galanin R1/GALR1

The neuropeptide galanin elicits a range of biological effects by interaction with specific G protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.

Long Name

Galanin Receptor Type 1

Entrez Gene IDs

2587 (Human); 14427 (Mouse); 50577 (Rat)

Alternate Names

GAL1; Galanin R1; galanin receptor 1; galanin receptor type 1; GalaninR1; GALNR1; GALNRGALNR1GAL1-R; GALR1; GALR-1