Human Galanin R2/GALR2 Antibody

Catalog #: MAB6544 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6544
MAB6544-SP

Detection of Galanin R2/GALR2 in HEK293 Human Cell Line Transfected with Human Galanin R2/GALR2 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galanin R2/GALR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GAL-R2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 616520

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Galanin R2
Accession # O43603

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Galanin R2/GALR2 in HEK293 Human Cell Line Transfected with Human Galanin R2/GALR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Galanin R2/GALR2 and eGFP was stained with either (A) Mouse Anti-Human Galanin R2/GALR2 Monoclonal Antibody (Catalog # MAB6544) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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Galanin R2 in Human Brain. Galanin R2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human Galanin R2 Monoclonal Antibody (Catalog # MAB6544) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells of blood vessels. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galanin R2/GALR2

GALR2 (Galanin Receptor 2) is one of three 54-60 kDa G-protein coupled multipass transmembrane receptors for the Galanin neuropeptide. Galanin is co-expressed with and modulates noradrenaline and serotonin systems. GALR2 is thought to modulate growth and apoptosis. Unlike GALR and GALR3, it is thought to be anti-depressive. GALR2 mRNA is found mainly in the central nervous system (except for cerebral cortex), but also in intestine, heart, kidney, liver and many cancers. The combined extracellular domains of human GALR2 share 82% and 86% amino acid identity with mouse and rat GALR2, respectively.

Long Name

Galanin Receptor Type 2

Entrez Gene IDs

8811 (Human); 29234 (Rat)

Alternate Names

GAL2R; GAL2-R; Galanin R2; galanin receptor 2; galanin receptor type 2; GalaninR2; GALNR2; GALNR2MGC125984; GALR2; GALR-2; MGC125983