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Human Galectin-10 Antibody

Catalog #: AF5447
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5447
AF5447-SP
Detection of Human Galectin‑10 by Western Blot.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galectin-10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Galectin-10 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Galectin‑10
Ser2-Arg142
Accession # Q05315
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Galectin-10 antibody by Western Blot. View Larger

Detection of Human Galectin‑10 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Intracellular Staining by Flow Cytometry View Larger

Detection of Galectin‑10 in Human Tregs by Flow Cytometry. Human Tregs were expanded from PBMC using Cloudz Human Treg Expansion Kit (CLD006) then stained with (A) Goat Anti-Human Galectin-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) or (B) Goat IgG Control Antibody (AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107) and Mouse Anti-Human CD25/IL-2 Ra Allophycocyanin-conjugated Monoclonal Antibody (FAB1020A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Intracellular Staining by Flow Cytometry Detection of Galectin-10 antibody in Human PBMC lymphocytes antibody by Flow Cytometry. View Larger

Detection of Galectin‑10 in Human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Goat Anti-Human Galectin-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) followed by CFS-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109) and Mouse Anti-Human CD25/IL-2 Ra Allophycocyanin-conjugated Monoclonal Antibody (Catalog # FAB1020A).Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-10

Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one Galectin domain (aa 6‑138) that contains two dimerization motifs (aa 6‑10 and 131‑135). Two molecular weight isoforms of 15 and 14 kDa have been described that are not yet characterized. There is no known structural rodent counterpart to human Galectin-10.

Entrez Gene IDs
1178 (Human)
Alternate Names
Charcot-Leyden crystal proteinLGALS10A; CLC; EC 3.1.1.5; eosinophil lysophospholipase; GAL10; Gal-10; galectin 10; Galectin10; Galectin-10; LGALS10; LPPL_HUMAN; Lysolecithin acylhydrolase; MGC149659

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Citations for Human Galectin-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Effects of Neutrophil and Eosinophil Extracellular Trap Formation on Refractoriness in Chronic Rhinosinusitis With Nasal Polyps
    Authors: Hyunkyung Cha, Hee-Suk Lim, Jin-A Park, Ara Jo, Hyun Tae Ryu, Do Won Kim et al.
    Allergy, Asthma & Immunology Research
  2. Galectin-10, a potential biomarker of eosinophilic airway inflammation.
    Authors: Chua, Justin C, Douglass, Jo A, Gillman, Andrew
    PLoS ONE, 2012-08-06;7(8):e42549.
    Species: Human
    Sample Types: Sputum
    Applications: Western Blot

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