Human GBX2 Antibody

Name: Human GBX2 Antibody
Brand: R&D Systems
SKU: AF4638
Price: 410 USD

Catalog #: AF4638
2 Citations Datasheet / COA / SDS

Discontinued Product

AF4638 has been discontinued.

View all GBX2 products.

Detection of Human GBX2 by Western Blot.

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All GBX2 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GBX2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GBX2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GBX2
Lys126-Asn247
Accession # P52951

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GBX2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human GBX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GBX2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GBX2

GBX2 (gastrulation brain homeobox) is a member of the homeobox family, which shares a 180 bp DNA sequence that encodes the DNA binding homeodomain. GBX2, along with OTX2 and FGF8 establish midbrain-hindbrain boundries in the developing brain. Increased GBX2 expression has also been associated with pluripotent cells and metastasic potential in cancers.

Long Name

Gastrulation Brain Homeobox 2

Entrez Gene IDs

2637 (Human); 14472 (Mouse); 114500 (Rat)

Alternate Names

Gastrulation and brain-specific homeobox protein 2; gastrulation brain homeo box 2; gastrulation brain homeobox 2; GBX2; homeobox protein GBX-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human GBX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures
Authors: JC Cossec, T Traboulsi, S Sart, Y Loe-Mie, M Guthmann, IA Hendriks, I Theurillat, ML Nielsen, ME Torres-Pad, CN Baroud, A Dejean
Cell Reports, 2023-04-15;42(4):112380.
Generation of thalamic neurons from mouse embryonic stem cells
Authors: A Shiraishi, K Muguruma, Y Sasai
Development, 2017-02-20;0(0):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC