Human GDNF Quantikine ELISA Kit

Name: Human GDNF Quantikine ELISA Kit
Brand: R&D Systems
SKU: DGD00
Rating: 5 (1 reviews)

Catalog #: DGD00
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DGD00

Control Products Available

QC253: Quantikine Immunoassay Control Set 991 for Human GDNF

Ancillary Products Available

DY010: Sample Activation Kit 1

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All GDNF ELISAs

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (1)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human GDNF Quantikine ELISA Kit Summary

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.696 pg/mL

Assay Range

4.7 - 300 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human GDNF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine^® Human GDNF Immunoassay is a 4.0 hour solid phase ELISA designed to measure human GDNF levels in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant human GDNF and antibodies raised against the recombinant protein. Results obtained for naturally occurring human GDNF showed linear curves that were parallel to the standard curves obtained using the Quantikine^® Human GDNF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human GDNF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	30.1	92.2	183	29.7	88.2	178
Standard Deviation	0.727	1.38	3.4	1.82	5.16	10.3
CV%	2.4	1.5	1.9	6.1	5.8	5.9

Recovery

The recovery of human GDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	85-112
Cell Lysis Buffer (n=4)	99	93-107
EDTA Plasma (n=5)	81	65-91
Heparin Plasma (n=5)	84	69-95
Serum (n=5)	86	72-99

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human GDNF were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human GDNF Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: GDNF

GDNF (Glial Cell Line-derived Neurotrophic Factor) is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. It is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF binding to GFR alpha 1 induces the recruitment of Ret, NCAM-1/CD56, various integrins, Syndecan-3, or N-Cadherin. GDNF-based therapies show promise in several neurodegenerative disorders, particularly Parkinson’s disease.

Long Name:

Glial Cell line-derived Growth Factor

Entrez Gene IDs:

2668 (Human); 25453 (Rat)

Alternate Names:

Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3

Related Research Areas

Citation for Human GDNF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

GDNF/GFRA1 signaling contributes to chemo- and radioresistance in glioblastoma
Authors: Avenel, ICN;Ewald, JD;Ariey-Bonnet, J;Kristensen, IH;Petterson, SA;Thesbjerg, MN;Burton, M;Thomassen, M;Wennerberg, K;Michaelsen, SR;Kristensen, BW;
Scientific reports
Species: Human
Sample Types: Cell Culture Supernates