Human GFR alpha -like Antibody

Catalog #: MAB9697 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9697-100
MAB9697-SP

Detection of GFR alpha -like in HEK293 Human Cell Line Transfected with Human GFR alpha -like by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GFR alpha -like Antibody Summary

Species Reactivity

Human

Specificity

Detects human GFR alpha ‑like in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2345C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human GFR alpha ‑like
Ser19-Glu351
Accession # Q6UXV0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA binding assay, 100-800 ng/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human GDF-15 (Catalog # 9279-GD) to immobilized Recombinant Human GFR alpha -like Fc Chimera (Catalog # 9697-GR) coated at 0.1 μg/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GFR alpha -like in HEK293 Human Cell Line Transfected with Human GFR alpha -like by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GFRa-like (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Rabbit Anti-Human GFRa-like Monoclonal Antibody (Catalog # MAB9697) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL respectively. It is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling (2-4). GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

References