Human Ghrelin/Obestatin Antibody

Catalog #: MAB81492 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB81492-100
MAB81492-SP

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ghrelin/Obestatin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ghrelin/Obestatin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 226710

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human Ghrelin/Obestatin
Accession # Q9UBU3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Ghrelin/Obestatin Monoclonal Antibody (Catalog # MAB81491).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ghrelin/Obestatin

Mature Ghrelin peptides are the result of the clavage of the Ghrelin/Obestatin Prepropeptide, a 117 aa precursor peptide that is processed into three chains, Ghrelin-27 (aa 24-50), Ghrelin-28 (aa24-51) and Obestatin (aa76-98). The prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases. Mature Ghrelin is acylated with an N-octanoyl group on serine 3 which is required for receptor binding. Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa. Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs. Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin.

Entrez Gene IDs

51738 (Human)

Alternate Names

appetite-regulating hormone; ghrelin; ghrelin/obestatin preprohormone; ghrelin/obestatin prepropeptide; Ghrelin/Obestatin; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; MTLRPgrowth hormone secretagogue receptor ligand; obestatin; Protein M46