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Human Girdin Antibody

Catalog #: AF5345
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5345
AF5345-SP
Detection of Human Girdin by Western Blot.
2 Images
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Girdin Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human Girdin in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Girdin
Asn1510-Leu1824
Accession # Q3V6T2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
A431 human epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Girdin antibody by Western Blot. View Larger

Detection of Human Girdin by Western Blot. Western blot shows lysates of H69AR human small cell lung cancer cell line, MRC-5 human embryonic lung fibroblast cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Girdin at approximately 200 to 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human Girdin by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Girdin at approximately 206 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Girdin

Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.

Long Name
Girders of Actin Filament
Entrez Gene IDs
55704 (Human); 108686 (Mouse); 305605 (Rat)
Alternate Names
Akt phosphorylation enhancer; APE; APEGIRDIN; CCDC88A; coiled-coil domain containing 88A; Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein; G{alpha}-interacting vesicle-associated protein; Girders of actin filament; Girdin; GIV; GIVFLJ10392; GRDN; GRDNAKT-phosphorylation enhancer; HkRP1; HkRP1Galpha-interacting vesicle-associated protein; Hook-related protein 1; KIAA1212DKFZp686D0630

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Citations for Human Girdin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Girdin Promotes Tumorigenesis and Chemoresistance in Lung Adenocarcinoma by Interacting with PKM2
    Authors: Fuyang Cao, Desong Yang, Feiyu Tang, Can Lu, Xiang He, Songming Chen et al.
    Cancers (Basel)
  2. Girdin/GIV regulates collective cancer cell migration by controlling cell adhesion and cytoskeletal organization
    Authors: Xiaoze Wang, Atsushi Enomoto, Liang Weng, Yasuyuki Mizutani, Shaniya Abudureyimu, Nobutoshi Esaki et al.
    Cancer Science
  3. Negative regulation of amino acid signaling by MAPK-regulated 4F2hc/Girdin complex
    Authors: Liang Weng, Yi-Peng Han, Atsushi Enomoto, Yasuyuki Kitaura, Shushi Nagamori, Yoshikatsu Kanai et al.
    PLOS Biology
  4. Akt-Girdin signaling in cancer-associated fibroblasts contributes to tumor progression.
    Authors: Yamamura Y, Asai N, Enomoto A, Kato T, Mii S, Kondo Y, Ushida K, Niimi K, Tsunoda N, Nagino M, Ichihara S, Furukawa K, Maeda K, Murohara T, Takahashi M
    Cancer Res, 2015-03-01;75(5):813-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA.
    Authors: Ota, Haruko, Hikita, Takao, Sawada, Masato, Nishioka, Tomoki, Matsumoto, Mami, Komura, Masayuki, Ohno, Akihisa, Kamiya, Yukiyo, Miyamoto, Takuya, Asai, Naoya, Enomoto, Atsushi, Takahashi, Masahide, Kaibuchi, Kozo, Sobue, Kazuya, Sawamoto, Kazunobu
    Nat Commun, 2014-07-30;5(0):4532.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation.
    Authors: Tomiyama, L, Sezaki, T, Matsuo, M, Ueda, K, Kioka, N
    Oncogene, 2014-03-24;34(9):1141-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain.
    Authors: Ota H, Hikita T, Nishioka T, Matsumoto M, Ito J, Asai N, Enomoto A, Takahashi M, Kaibuchi K, Sobue K, Sawamoto K
    Biochem Biophys Res Commun, 2013-11-06;442(1):16-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Girdin and its phosphorylation dynamically regulate neonatal vascular development and pathological neovascularization in the retina.
    Authors: Ito T, Komeima K, Yasuma T, Enomoto A, Asai N, Asai M, Iwase S, Takahashi M, Terasaki H
    Am J Pathol, 2012-11-27;182(2):586-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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