Human GKAP/DLGAP1 Antibody

Catalog #: MAB7296 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7296
MAB7296-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GKAP/DLGAP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GKAP in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 727425

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GKAP
Lys368-Glu472
Accession # O14490

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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GKAP in Human Brain. GKAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human GKAP Monoclonal Antibody (Catalog # MAB7296) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GKAP/DLGAP1

GKAP, also known as DAP1, DLGAP, and SAPAP1, is an approximately 45 kDa cytoplasmic membrane associated protein. GKAP is a component of a neuronal postsynaptic complex that also includes PSD-95 and SHANK. This complex links neurotransmitter channels with the cytoskeleton and can promote NMDA R channel opening. Alternate splice forms of human GKAP have N-terminal and/or C-terminal truncations and are differentially expressed during brain development. Within aa 368-472 human, mouse, and rat GKAP share 100% aa sequence identity.

Long Name

Guanylate Kinase-associated Protein

Entrez Gene IDs

9229 (Human); 224997 (Mouse); 65040 (Rat)

Alternate Names

DAP1; DAP-1-ALPHA; DAP-1DAP-1-BETA; discs, large (Drosophila) homolog-associated protein 1; disks large-associated protein 1; DLGAP1; GKAP; GKAPMGC88156; hGKAP; PSD-95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD-95-associated protein 1; SAPAP1; SAPAP1Guanylate kinase-associated protein