Human GKAP/DLGAP1 Antibody

Catalog #: AF7296 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7296
AF7296-SP

Detection of Human GKAP/DLGAP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GKAP/DLGAP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GKAP/DLGAP1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DLGAP2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GKAP/DLGAP1
Lys368-Glu472
Accession # O14490

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GKAP/DLGAP1 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GKAP/DLGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7296) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific band were detected for GKAP/DLGAP1 at approximately 80 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GKAP/DLGAP1

DLGAP1 (Disks Large-associated protein 1; also SAPAP1, GKAP and SAP90 binding protein) is an intracellular, 117-130 kDa member of the SAPAP family of proteins. It is widely expressed (astrocytes, neurons, fibroblasts and epithelium), and serves as a platform for dynein-DGL1 interaction. This is important during cell migration where the centrosome must be positioned between the nucleus and direction of migration in response to microtubule formation under the protruding edge of the cell membrane. DLGAP1 first binds DLG-1 and then recruits dynein, with a resultant interaction that allows for microtubule anchoring and centrosome positioning. Human DLGAP1 is 977 amino acids (aa) in length. It contains multiple 14 aa acidic repeats, three Pro-rich segments, and at least 14 potential phosphorylation sites. Multiple potential splice variants are reported. In various combinations, there may be a 17, 27 and 31 aa substitution for aa 1-319, a deletion of either aa 531-540 or 531-558, a 24 aa substitution for aa 1-318, and a 13 aa substitution for aa 531-977. Most appear to generate 70-80 kDa isoforms in SDS-PAGE. Over aa 368-472, human and mouse DLGAP1 are identical in aa sequence.

Long Name

Guanylate Kinase-associated Protein

Entrez Gene IDs

9229 (Human); 224997 (Mouse); 65040 (Rat)

Alternate Names

DAP1; DAP-1-ALPHA; DAP-1DAP-1-BETA; discs, large (Drosophila) homolog-associated protein 1; disks large-associated protein 1; DLGAP1; GKAP; GKAPMGC88156; hGKAP; PSD-95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD-95-associated protein 1; SAPAP1; SAPAP1Guanylate kinase-associated protein