Human GLI-3 Antibody

Catalog #: MAB3690 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB3690-100
MAB3690-SP

Detection of GLI-3 in Human SW480 cell line.

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Human GLI-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GLI-3 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2352B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

3-25 µg/mL

Immersion fixed SW480 human fibroblast carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of GLI-3 in Human SW480 cell line. GLI‑3 was detected in immersion fixed SW480 fibroblast carcinoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Rabbit Anti-Human GLI‑3 Monoclonal Antibody (Catalog # MAB3690) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-3

GLI3 is a zinc finger protein. The GLI family of proteins are transcription factors and are mediators of Sonic hedgehog signaling. GLI3 is known to be a transcriptional repressor but also may have a positive transcription function. Mutations in the GLI3 gene are associated with many polydactyly diseases.

References

Rupert JM., Vogelstein B., Arheden K., Kinzler KW., "GLI3 Encodes a 190-kilodalton Protein with Multiple Regions of GLI Similarity". Molecular and Cellular Biology. 1990 Oct; 10(10): 5408-15. Taipale J., Beachy PA., "The Hedgehog and Wnt Signaling Pathways in Cancer". Nature. 2001 May;411(6835): 349-54. Rash BG., Grove EA., "Patterning the Dorsal Telencephalon: a Role for Sonic Hedgehog ?". The Journal of Neuroscience. 2007 Oct; 27(43): 11595-603.

Long Name

GLI-Kruppel family member GLI3

Entrez Gene IDs

2737 (Human); 14634 (Mouse); 140588 (Rat)

Alternate Names

ACLS; ACLSPAPAPHS; ADD; Bph; GCPS; GLI family zinc finger 3; GLI3 form of 190 kDa; GLI3 full length protein; GLI3; GLI-3; GLI3-190; GLI3FL; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3; Greig cephalopolysyndactyly syndrome; oncogene GLI3; PAP-A; PAPA1; PAPB; PAPBDNA-binding protein; Pdn; PHS; PPDIV; Xt; zinc finger protein GLI3