Human Glucagon R/GCGR Antibody

Catalog #: MAB10296 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10296-100
MAB10296-SP

Detection of Glucagon R/GCGR in HEK293 Human Cell Line Transfected with Human Glucagon R/GCGR and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glucagon R/GCGR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glucagon R/GCGR in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 591929

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Glucagon R/GCGR
Met1-Gln142
Accession # P47871

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Glucagon R/GCGR in HEK293 Human Cell Line Transfected with Human Glucagon R/GCGR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Glucagon R/GCGR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glucagon R/GCGR Monoclonal Antibody (Catalog # MAB10296) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glucagon R/GCGR

GLR (GCGR/Glucagon Receptor) is a GPCR for glucagon that plays a role in blood glucose levels and glucose homeostasis. Glucagon receptors are mainly expressed in liver and kidney but also expressed in other tissues such as heart, adipose, spleen, thymus, adrenal glands, pancreas, cerebral cortex and the gastrointestinal tract. Mutations in the GCGR gene is associated with Type 2 Diabetes Mellitus and inactivation of the receptor causes glucagon resistance. GCGR is found on the cell membrane but is rapidly internalized following ligand-binding via endocytosis.

Long Name

Glucagon Receptor

Entrez Gene IDs

2642 (Human); 14527 (Mouse)

Alternate Names

FLJ97182; GCGR; GGR; GLR; GL-R; Glucagon R; glucagon receptor; GlucagonR; MGC138246