Human GluR3 Antibody

Catalog #: MAB11329 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11329-100
MAB11329-SP

Detection of GluR3 in TT (positive) and THP‑1 (negative) cells.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GluR3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GluR3 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1059821

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # P42263

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-25 µg/mL

TT human medullary thyroid cancer cell line (positive) and THP‑1 human acute monocytic leukemia cell line (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of GluR3 in TT (positive) and THP‑1 (negative) cells. GluR3 was detected in immersion fixed TT human medullary thyroid cancer cell line (positive) and absent in THP‑1 human acute monocytic leukemia cell line (negative) using Mouse Anti-Human GluR3 Monoclonal Antibody (Catalog # MAB11329) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GluR3

GluR3 is a receptor for glutamate. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiological processes. This gene belongs to a family of AMPA receptors. GluR3 is a gene product of the GRIA3 gene and its pre-mRNA is subject to RNA editing. In humans, 80-90% of GRIA3 transcripts are edited. This allows sustained response to rapid stimuli. There is a tentative link between defective GRIA3 variants and a highly elevated risk of schizophrenia.

References

Entrez Gene: GRIA3 glutamate receptor, ionotropic, AMPA 3.
Seeburg PH, Higuchi M, Sprengel R. "RNA Editing of Brain Glutamate Receptor channels: Mechanism and Physiology". Brain Res. Brain Res. Rev. 1998, May; 26(2-3):217.
Lomeli H, Mosbacher J, Höger T, Geiner JR, Kuner T, Monyer H, Higuchi M, Bach A, Seeburg PH., "Control of Kinetic Properties of AMPA rREceptor Channels by Nuclear RNA Editing". Science. 1994, Dec; 266(5191):1709.

Long Name

Glutamate Receptor 3

Entrez Gene IDs

2892 (Human); 53623 (Mouse); 29628 (Rat)

Alternate Names

AMPA 3; AMPA3; AMPA-selective glutamate receptor 3; GluA3; GluR3; GluR-3; GLURC; GLUR-C; GLUR-K3; glutamate receptor C; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; GRIA3