Human GluR4 Antibody

Catalog #: MAB11363 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11363-100
MAB11363-SP

Detection of GluR4 in Transfected HEK293 Cell Line (Positive) and Wild Type HEK293 Cell Line (Negative).

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All GluR4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GluR4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GluR4 in direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2913A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human GluR4
Met112-His314
Accession # P48058.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

3-15 µg/mL

Immersion fixed Transfected HEK293 Human Embryonic Kidney Cell Line (Positive) and absent in Wild Type HEK293 Human Embryonic Kidney Cell Line (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of GluR4 in Transfected HEK293 Cell Line (Positive) and Wild Type HEK293 Cell Line (Negative). GluR4 was detected in immersion fixed Transfected HEK293 Human Embryonic Kidney Cell Line (Positive) and absent in Wild Type HEK293 Human Embryonic Kidney Cell Line (Negative) using Rabbit Anti-Human GluR4 Monoclonal Antibody (Catalog # MAB11363) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GluR4

Ionotropic glutamate receptor 4 (GluR4) is a 100.9 kDa protein that is part of a family (iGluR 1-7) of ligand-gated ion channels that mediates fast transmission of neurotransmitter signals in neuronal cells. These receptors respond to several agonists including N-methyl-d-aspartic acid (NMDA), alpha -amino-3-hydroxyl-5-methylisoxazole-4-proprionic acid (AMPA), or kainic acid (KA). iGluRs are tetrameric structures made by the dimerization of dimers. These receptors can form through a combination of iGluR1-iGluR4 subunits. Mature human GluR4 is a 902 amino acid (aa) molecule, and alternate gene-splicing results in transcript variations encoding different isoforms that may effect their signal transduction properties. GluR4 is made up of an extracellular amino-terminal domain (ATD), ligand binding domain (LBD), a common pore-forming transmembrane domain (TMD) and an intracellular C-terminal domain (CTD). GluR4's clam-shell shaped LBD binds to agonists. The LBD closes upon ligand binding to induce a conformational change to the TMD, resulting in the ion channel opening. GluR4 is highly expressed in areas that utilize fast kinetics and rapid desensitization. Examples of GluR4 expression include tissues of the central cervical nucleus in rats, the outer plexiform layer of goldfish, the retinal cells of chicken embryos and the human cerebral cortex.

Long Name

Glutamate Receptor 4

Entrez Gene IDs

2893 (Human); 14802 (Mouse); 29629 (Rat)

Alternate Names

AMPA-selective glutamate receptor 4; GluA4; GluR4; gluR-4; GLUR4C; GLUR4gluR-D; GLURD; GluR-D; glutamate receptor 4; Glutamate receptor ionotropic, AMPA 4; glutamate receptor, ionotrophic, AMPA 4