Human GluR7/GRIK3 Antibody

Catalog #: MAB11328 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11328-100
MAB11328-SP

Detection of GluR7/GRIK3 in Human Hippocampus.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GluR7/GRIK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GluR7/GRIK3 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1059901

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GluR7/GRIK3 containing peptide.
Accession # Q13003

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Hippocampus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of GluR7/GRIK3 in Human Hippocampus. GluR7/GRIK3 was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human GluR7/GRIK3 Monoclonal Antibody (Catalog # MAB11328) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GluR7/GRIK3

GluR7 is a receptor for glutamate. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain. This gene can co-assemble with GRIK4 or GRIK5 to form heteromeric receptors. GluR7 is one of the five subtypes of kainate receptors. Kainate receptors are widely expressed in the CNS and are involved in the regulation of transmitter release, synapse formation, and in the pathophysiology of brain diseases. Genetic variants in the GluR7 gene are associated with schizophrenia, major depression, and bipolar disorder.

References

Entrez Gene: GRIK3 glutamate receptor, ionotropic, kainate 3.
Matute C. "Therapeutic Potential of Kainate Receptors". CNS Neuroscience & Therapeutics. 2011 Dec; 17(6):661.
Begni S, Popoli M, Moraschi S, Bignotti S, Tura G.B, Gennarelli M., "Association Between the Ionotropic Glutamate Receptor Kainate 3 (GRI3) Ser310Ala Polymorphism and Schizophrenia". Mol Psychiatry. 2002; 7(4):416.

Long Name

Glutamate Receptor 7

Entrez Gene IDs

2899 (Human); 14807 (Mouse); 298521 (Rat)

Alternate Names

EAA5; Excitatory amino acid receptor 5; GLR7; GluK3; GluR7; gluR-7; GluR7a; GLUR7dJ1090M5.1 (glutamate receptor, ionotropic, kainate 3 (GLUR7)); Glutamate receptor 7; glutamate receptor, ionotropic kainate 3; glutamate receptor, ionotropic, kainate 3; GRIK3