Human Glut1 Antibody Summary
Met1-Val492
Accession # AAA52571
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Glut1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Glut1 by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glut1
Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑5). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.
- Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
- Meuckler, M. et al. 1985, Science 229:941.
- Jones, K.S. et al. 2006, J. Virol. 8291.
- Takenouchi, N. et al. 2007, J. Virol. 1506.
- Kinet, S. et al. 2007, Retrovirology 4:31.
Product Datasheets
Citations for Human Glut1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Metformin suppresses Nrf2-mediated chemoresistance in hepatocellular carcinoma cells by increasing glycolysis
Authors: Liangyu Cai, Xin Jin, Jiannan Zhang, Le Li, Jinfeng Zhao
Aging (Albany NY)
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The circadian clock regulates RPE-mediated lactate transport via SLC16A1 (MCT1)
Authors: Nemanja Milićević, Jacoline B. ten Brink, Anneloor L.M.A. ten Asbroek, Arthur A. Bergen, Marie-Paule Felder-Schmittbuhl
Experimental Eye Research
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