Human Glut2 PE-conjugated Antibody

Catalog #: FAB1414P
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1414P

Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry.

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All Glut2 Antibodies

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut2 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Recognizes human Glut2 expression on human Glut2‑transfected NS0 cells, but not on control transfectants. Was shown to detect Glut2 on the surface of the human intestinal cell line Caco2 (2) in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with human Glut1 or human Glut3.

Source

Monoclonal Mouse IgG_2A Clone # 199017

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Glut2
Met1-Val524
Accession # P11168

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut2 PE-conjugated Monoclonal Antibody (Catalog # FAB1414P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney, and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1).

References

Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
Mahraoui, L. et al. (1994) J. Biochem. 298:629.

Long Name

Glucose Transporter Type 2

Entrez Gene IDs

6514 (Human); 20526 (Mouse)

Alternate Names

GLUT2 Glucose transporter type 2, liver; Glut2; GLUT-2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human Glut2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression
Authors: Ana F. Brito, Ana M. Abrantes, Marina Ribeiro, Rui Oliveira, João Casalta-Lopes, Ana C. Gonçalves et al.
Journal of Clinical and Experimental Hepatology
Increase in the Expression of Glucose Transporter 2 (GLUT2) on the Peripheral Blood Insulin-Producing Cells (PB-IPC) in Type 1 Diabetic Patients after Receiving Stem Cell Educator Therapy
Authors: Zhao, Y;Veysman, B;Antolijao, K;Zhao, Y;Papagni, Y;Wang, H;Ross, R;Tibbot, T;Povrzenic, D;Fox, R;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Understanding the Role of GLUT2 in Dysglycemia Associated with Fanconi-Bickel Syndrome
Authors: S Sharari, B Kabeer, I Mohammed, B Haris, I Pavlovski, I Hawari, AA Bhat, M Toufiq, S Tomei, R Mathew, N Syed, S Nisar, S Maacha, JC Grivel, D Chaussabel, J Ericsson, K Hussain
Biomedicines, 2022-08-29;10(9):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Generation of Multipotent Stem Cells from Adult Human Peripheral Blood Following the Treatment with Platelet-Derived Mitochondria
Authors: H Yu, W Hu, X Song, Y Zhao
Cells, 2020-05-29;9(6):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Presence of diabetes autoantigens in extracellular vesicles derived from human islets
Authors: CP Hasilo, S Negi, I Allaeys, N Cloutier, AK Rutman, M Gasparrini, É Bonneil, P Thibault, É Boilard, S Paraskevas
Sci Rep, 2017-07-10;7(1):5000.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Mutations in SLC2A2 gene reveal hGLUT2 function in pancreatic beta cell development.
Authors: Michau A, Guillemain G, Grosfeld A, Vuillaumier-Barrot S, Grand T, Keck M, L'Hoste S, Chateau D, Serradas P, Teulon J, De Lonlay P, Scharfmann R, Brot-Laroche E, Leturque A, Le Gall M
J Biol Chem, 2013-08-28;288(43):31080-92.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry