Human Glutathione S‑Transferase mu 1/
GS Antibody

Catalog #: AF6894 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6894
AF6894-SP

Detection of Human Glutathione S‑Transferase mu 1/ GS by Western Blot.

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All Glutathione S-Transferase mu 1/GSTM1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glutathione S‑Transferase mu 1/
GS Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glutathione S‑Transferase mu 1/GS in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Glutathione S‑Transferase mu 1/GS
Met1-Lys218
Accession # P09488

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Glutathione S‑Transferase mu 1/ GS by Western Blot. Western blot shows lysates of human kidney tissue, human adrenal gland tissue, human stomach tissue, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Glutathione S-Transferase mu 1/GS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glutathione S-Transferase mu 1/GS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase mu 1/GSTM1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).

References

Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
Mo, Z. et al. (2009) Prostate. 69:662.
Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.

Entrez Gene IDs

2944 (Human)

Alternate Names

EC 2.5.1.18; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; Glutathione STransferase mu 1; Glutathione S-Transferase mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; HB subunit 4; H-B; MGC26563; MU; MU-1; S-(hydroxyalkyl)glutathione lyase