Human Glycophorin A PE-conjugated Antibody

Catalog #: FAB12281P
2 Citations Datasheet / COA / SDS

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FAB12281P

Detection of Glycophorin A in TF-1 Human Cell Line by Flow Cytometry.

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Citations (2)

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Human Glycophorin A PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].

Source

Monoclonal Mouse IgG₁ Clone # R10

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Abelson mouse leukemia virus-induced human pre-B tumor cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Glycophorin A in TF-1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Glycophorin A PE-conjugated Monoclonal Antibody (Catalog # FAB12281P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glycophorin A

Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).

References

Greaves, M.F. et al. (1983) Blood 61(4):645.

Entrez Gene IDs

2993 (Human)

Alternate Names

CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR

Product Datasheets

Product Datasheet

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Citations for Human Glycophorin A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
Authors: A Borojevi?, A Jaukovi?, T Kukolj, S Mojsilovi?, H Obradovi?, D Trivanovi?, M Živanovi?, Ž Ze?evi?, M Simi?, B Gobelji?, D Vuji?, D Bugarski
Biomolecules, 2022-02-18;12(2):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Targeted erythropoietin selectively stimulates red blood cell expansion in vivo.
Authors: DR Burrill, Burrill D, A Vernet, Vernet A, JJ Collins, PA Silver, JC Way, Way J
Proc Natl Acad Sci U S A, 2016-04-25;113(19):5245-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry