Human gp130 Antibody Summary
Leu24-Glu619 (Glu619Asp)
Accession # P40189
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Human gp130 ELISA Standard Curve. Recombinant Human gp130 protein was serially diluted 2-fold and captured by Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB2281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: gp130
Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).
- Wang, Y. et al. (1992) Genomics 14:666.
- Muller-Newen, G. (2003) Sci. STKE pe40.
- Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
- Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
- Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
Product Datasheets
Citations for Human gp130 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
Filter your results:
Filter by:
-
Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma
Authors: Karavyraki M, Porter RK.
Medical Oncology
-
Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma
Authors: Karavyraki M, Porter RK.
Medical Oncology
-
Targeting EphA2 suppresses hepatocellular carcinoma initiation and progression by dual inhibition of JAK1/STAT3 and AKT signaling
Authors: H Wang, W Hou, A Perera, C Bettler, JR Beach, X Ding, J Li, MF Denning, A Dhanarajan, SJ Cotler, C Joyce, J Yin, F Ahmed, LR Roberts, W Qiu
Cell Reports, 2021-02-23;34(8):108765.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
IL-27 Modulates Chemokine Production in TNF-? -Stimulated Human Oral Epithelial Cells
Authors: Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo
Cell. Physiol. Biochem., 2017-10-05;43(3):1198-1206.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Trans-signaling is a dominant mechanism for the pathogenic actions of interleukin-6 in the brain.
Authors: Campbell I, Erta M, Lim S, Frausto R, May U, Rose-John S, Scheller J, Hidalgo J
J Neurosci, 2014-02-12;34(7):2503-13.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle.
Authors: Ammit AJ, Moir LM, Oliver BG, Hughes JM, Alkhouri H, Ge Q, Burgess JK, Black JL, Roth M
Am. J. Physiol. Lung Cell Mol. Physiol., 2006-08-25;292(1):L199-206.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human gp130 Antibody
There are currently no reviews for this product. Be the first to review Human gp130 Antibody and earn rewards!
Have you used Human gp130 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
