Human GPR108 Antibody

Catalog #: MAB11128 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11128-100
MAB11128-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR108 Antibody Summary

Species Reactivity

Human

Source

Monoclonal Mouse IgG_2A Clone # 1047612

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GPR108 synthetic peptide
Accession # Q9NPR9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Prostate

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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GPR108 in Human Prostate. GPR108 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human GPR108 Monoclonal Antibody (Catalog # MAB11128) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot

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Detection of Human GPR108 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line (wild type) and HEK293T human embryonic kidney cell line transfected with human GPR108. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR108 Monoclonal Antibody (Catalog # MAB11128) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR108 at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR108

Human GPR108, also known as Lung seven transmembrane receptor 2, is a 543 amino acids long receptor belonging to the G-protein-coupled receptors (GPCRs) family of receptors. Human GPR108 may play a role in intracellular immune modulation by activating NF-kappaB response and attenuating Toll-like-receptor response (1). I has also been shown essential function in adeno-associated virus (AAV) entry to cells (2).

References