Human GPR116 Antibody

Catalog #: MAB112481 Datasheet / COA / SDS

Catalog #	Availability	Size / Price
MAB112481-SP	Contact your distributor for availability.	25 ug / Please Inquire
MAB112481-100	Contact your distributor for availability.	100 ug / Please Inquire

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Detection of GPR116 in HEK293 human cell line transfected with human GPR116 by Flow Cytometry.

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All GPR116 Antibodies

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Human GPR116 Antibody Summary

Species Reactivity

Human

Specificity

Detects Human GPR116 in Direct ELISAs. In Flow Cytometry, it detects human GPR116 in transfected cells, but not in non-transfected parental cell line.

Source

Monoclonal Mouse IgG₁ Clone # 1055815

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-expressed recombinant Human GPR116 extracellular domain.
His643-Asn945
Accession # Q8IZF2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

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Human GPR116 Alexa Fluor® 488-conjugated Antibody

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Applications

Recommended Concentration

Sample

Flow Cytometry

25 µg/mL

HEK293 human cell line transfected with human GPR116

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPR116 in HEK293 human cell line transfected with human GPR116 by Flow Cytometry. HEK293 human cell line transfected with human GPR116 was stained with either (A) Mouse Anti-Human GPR116 Monoclonal Antibody (Catalog # MAB112481) or (B) Mouse IgG1 Isotype Control (MAB002), followed by APC-conjugated anti-mouse IgG secondary antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR116

GPR116, also known as Adhesion G protein-coupled receptor F5 or ADGRF5, belongs to the LN-TM7 subfamily of the G protein-coupled receptor 2 family, also known as adhesion GPCRs. It exists as a highly glycosylated disulfide-linked dimer at the cell surface. GPR116 may have a role in the regulation of acid-base balance and is also being investigated for it's involvement in adipocyte biology.

Long Name

G Protein-coupled Receptor 116

Entrez Gene IDs

221395 (Human); 224792 (Mouse); 245977 (Rat)

Alternate Names

FLJ90640; G protein-coupled receptor 116; GPR116; Ig-Hepta homolog; KIAA0758DKFZp564O1923; KPG_001; probable G-protein coupled receptor 116