Human GPR15 APC-conjugated Antibody

Catalog #: FAB3654A
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3654A-025

Detection of GPR15 in HEK293 Human Cell Line Transfected with Human GPR15 and eGFP by Flow Cytometry.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR15 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human GPR15-transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 367902

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR15
Met1-Leu360
Accession # P49685

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPR15 in HEK293 Human Cell Line Transfected with Human GPR15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR15 and eGFP was stained with either (A) Mouse Anti-Human GPR15 APC-conjugated Monoclonal Antibody (Catalog # FAB3654A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR15

GPR15, also known as BOB, is a seven-transmembrane G protein-coupled receptor that is expressed in CD4⁺ T cells and alveolar macrophages. GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. Human GPR15 shares 96%‑100% amino acid sequence identity with chimpanzee, macaque, and rhesus GPR15, and 76% with mouse GPR15.

Long Name

G Protein-coupled Receptor 15

Entrez Gene IDs

2838 (Human); 71223 (Mouse)

Alternate Names

Bob; Brother of Bonzo; G protein-coupled receptor 15; GPR15; G-protein coupled receptor 15; MGC126828; MGC126830

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human GPR15 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Dietary L-Tryptophan consumption determines the number of colonic regulatory T cells and susceptibility to colitis via GPR15
Authors: Van, NT;Zhang, K;Wigmore, RM;Kennedy, AI;DaSilva, CR;Huang, J;Ambelil, M;Villagomez, JH;O'Connor, GJ;Longman, RS;Cao, M;Snook, AE;Platten, M;Kasenty, G;Sigal, LJ;Prendergast, GC;Kim, SV;
Nature communications
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

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