Human GPR22 Antibody

Catalog #: MAB4500 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4500
MAB4500-SP

Detection of GPR22 in A172 Human Cell Line by Flow Cytometry.

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All GPR22 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR22 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR22. Stains human GPR22 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 455108

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human GPR22
Met1-Asp433
Accession # Q99680

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of GPR22 antibody in A172 Human Cell Line antibody by Flow Cytometry.

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Detection of GPR22 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR22 Monoclonal Antibody (Catalog # MAB4500, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR22

GPR22 is a 7TM G protein-coupled receptor that is expressed in the brain. It maps to chromosome 7, region q22-q31.1. Human GPR22 shares 97% aa sequence identity with mouse GPR22.

Long Name

G Protein-coupled Receptor 22

Entrez Gene IDs

2845 (Human); 73010 (Mouse); 298944 (Rat)

Alternate Names

G protein-coupled receptor 22; GPR22; MGC129847; probable G-protein coupled receptor 22