Human GPR37 Antibody

Catalog #: MAB44501
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB44501-100
MAB44501-SP

Detection of Human GPR37 by Western Blot.

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All GPR37 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR37 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR37 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1036313

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human GPR37
Leu28-Met265
Accession # O15354

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Human brain (motor cortex) and K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GPR37 by Western Blot. Western blot shows lysates of human brain (motor cortex) and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR37 Monoclonal Antibody (Catalog # MAB44501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR37 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR37

GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 587 aa, 7-transmembrane receptor for the neuroprotective and glioprotective factor prosaposin (1, 4). It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus (2). It is a substrate of the E3 ubiquitin ligase, parkin, which is up-regulated during endoplasmic reticulum stress (3). In a juvenile form of Parkinson's disease, GPR37 accumulates, contributing to stress-induced neuronal cell death (2). The extracelluar domains (aa 27-265) of human and mouse GPR37 share 68% aa identity.

References

Marazziti, D. et al. (1997) Genomics, 45:68.
Donohue, P.J. et al. (1998) Brain Res Mol Brain Res. 54:152.
Omura, T. et al. (2006) J Neurochem. 99:1456.
Meyer, R.C. et al. (2013) Proc. Natl. Acad. Sci. U. S. A. 110: 9529

Long Name

G Protein-coupled Receptor 37

Entrez Gene IDs

2861 (Human); 14763 (Mouse); 117549 (Rat)

Alternate Names

EDNRBL; Endothelin B receptor-like protein 1; ETBR-LP-1; G protein-coupled receptor 37 (endothelin receptor type B-like); GPR37; hET(B)R-LP; PAELR; PAELRETBR-LP-1; Parkin-associated endothelin receptor-like receptor; probable G-protein coupled receptor 37

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Orphan G Protein-Coupled Receptors

Citation for Human GPR37 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1