Human Granzyme A APC-conjugated Antibody

Name: Human Granzyme A APC-conjugated Antibody
Brand: R&D Systems
SKU: IC29051A

Catalog #: IC29051A Datasheet / COA / SDS

Discontinued Product

IC29051A has been discontinued.

View all Granzyme A products.

Detection of Granzyme A in Human CD56+ Whole Blood Cells by Flow Cytometry.

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All Granzyme A Antibodies

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Human Granzyme A APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Granzyme A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Granzyme B, C, D, G, or H is observed.

Source

Monoclonal Mouse IgG_2B Clone # 356412

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Granzyme A
Cys26-Val262
Accession # P12544

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Granzyme A antibody in Human CD56+ Whole Blood Cells antibody by Flow Cytometry.

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Detection of Granzyme A in Human CD56+ Whole Blood Cells by Flow Cytometry. Human CD56+ whole blood cells were stained with Mouse Anti-Human Granzyme A APC-conjugated Monoclonal Antibody (Catalog # IC29051A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Granzyme A

Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1‑26), a propeptide (residues 27‑28) and a mature chain (residues 29‑262) (2). The purified recombinant human Granzyme A consists of residues 26 to 262. After being activated by lysyl endopeptidase, it cleaves a thioester substrate.

References

Lieberman, J. and Z. Fan (2003) Curr. Opin. Immunol. 15:553.
Gershenfeld, H.K. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1184.

Entrez Gene IDs

3001 (Human); 14938 (Mouse); 266708 (Rat)

Alternate Names

CTL Tryptase; CTLA3; CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease); Cytotoxic T-lymphocyte proteinase 1; EC 3.4.21; Fragmentin-1; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme A; Granzyme-1; GZMA; H factor; Hanukkah factor; HF; HFSP; HFSPEC 3.4.21.78