Human GRAP2 Antibody

Name: Human GRAP2 Antibody
Brand: R&D Systems
SKU: MAB4640
Rating: 3.5 (2 reviews)

Catalog #: MAB4640 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4640
MAB4640-SP

Detection of Human GRAP2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GRAP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human GRAP2 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 475804

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GRAP2
Met1-Arg330
Accession # O75791

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GRAP2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human GRAP2 Monoclonal Antibody (Catalog # MAB4640) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRAP2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRAP2

GRAP2 (GRB2 related adaptor protein 2), a divergent member of the GRB2/Sem5/Drk family, is an adaptor protein involved in leukocyte-specific protein-tyrosine kinase signaing. GRAP2 shares similar structural organization as GRB2 and GRAP with a central SH2 domain flanked by 2 SH3 domains. However, unlike GRB2 and GRAP, the SH2 domain and the C-terminal SH3 domain of GRAP2 contains a unique 120-amino acid glutamine/proline rich sequence. In T lymphocytes, GRAP2 interacts with LAT and SLP-76 to regulate NF-AT (nuclear factor of activated T cells) and form a signaling complex with HPK1 to mediate the JNK signaling pathway. GRAP2 also interacts with M-CSF R and the activated T cell co-stimulatory receptor CD28.

Long Name

GRB2-related Adaptor Protein 2

Entrez Gene IDs

9402 (Human); 17444 (Mouse); 366962 (Rat)

Alternate Names

Adapter protein GRID; GADS; GADSSH3-SH2-SH3 adapter Mona; GRAP2; GRB2L; GRB-2-like protein; GRB2-related adapter protein 2; GRB2-related adaptor protein 2; GRB2-related protein with insert domain; GRBLG; GRBLGGRAP-2; GRBX; Grf40 adapter protein; Grf40; grf-40; GRID; GRIDGRPL; Growth factor receptor-binding protein; growth factor receptor-bound protein 2-related adaptor protein 2; GRPL; Hematopoietic cell-associated adapter protein GrpL; hematopoietic cell-associated adaptor protein GRPL; Mona; P38; Protein GADS; SH3-SH2-SH3 adaptor molecule