Human GRB2 (SH2 Domain) Antibody Summary
Trp60-Glu152
Accession # P62993
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat GRB2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRB2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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GRB2 in Human Breast Cancer Tissue. GRB2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal and epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRB2
GRB2 (growth factor receptor-bound 2; also Ash) is a ubiquitously-expressed, 27 kDa member of the GRB2/sem-5 family of adaptor molecules. It serves as a linker for many intracellular proteins. Upon BCR ligation, LAB is phosphorylated, binds to GRB2, which subsequently recruits signaling factors. Upon InsR ligation, GRB2 binds to phosphorylated IRS-1, and serves as a linker to ras-activation. Human GRB2 is 217 amino acids (aa) in length. It contains one N-terminal SH3 domain (aa 3-54), a central SH2 domain (aa 60-152), and a C-terminal SH3 domain (aa 160-212). SH2 domains bind phosphotyrosine motifs; SH3 domains bind proline-rich regions. There is one splice form that shows a deletion of aa 60-100. This removes the SH2 domain and initiates apoptosis. Human GRB2 is over 99% aa identical to mouse and dog GRB2.
Product Datasheets
Citations for Human GRB2 (SH2 Domain) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Time-resolved multimodal analysis of Src Homology 2 (SH2) domain binding in signaling by receptor tyrosine kinases
Authors: JA Jadwin, D Oh, TG Curran, M Ogiue-Iked, L Jia, FM White, K Machida, J Yu, BJ Mayer
Elife, 2016-04-12;5(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Grb2 promotes integrin-induced focal adhesion kinase (FAK) autophosphorylation and directs the phosphorylation of protein tyrosine phosphatase alpha by the Src-FAK kinase complex.
Authors: Cheng S, Sun G, Schlaepfer D, Pallen C
Mol Cell Biol, 2013-11-18;34(3):348-61.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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