Human HAI-1 Antibody

Catalog #: MAB1048 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1048
MAB1048-SP

All HAI-1 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HAI-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HAI-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human HAI-2 or recombinant mouse HAI-1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 169417

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Val449
Accession # O43278

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human HAI‑1 (Catalog # 1048-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human HAI‑1 (Catalog # 1048-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-1

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

References

Denda, K. et al. (2002) J. Biol. Chem. 277:14053.
Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.

Long Name

HGF Activator Inhibitor Type 1

Entrez Gene IDs

6692 (Human); 20732 (Mouse)

Alternate Names

HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1