Human HAND1 Biotinylated Antibody

Catalog #: BAF3168 Datasheet / COA / SDS

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BAF3168

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Human HAND1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human human HAND1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human HAND2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HAND1
Met1-Gln215
Accession # O96004

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human HAND1

Immunocytochemistry

5-15 µg/mL

Immersion fixed SH‑SY5Y human neuroblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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HAND1 in SH‑SY5Y Human Cell Line. HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3168) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAND1

HAND1 belongs to the HAND family of conserved basic helix-loop-helix transcription factors. It is expressed in the heart, ganglia, neural crest derivatives and extraembryonic tissues. HAND1 plays an essential role in heart development and is critical for the specification of extraembryonic tissues including trophoblasts. Human and mouse HAND1 share approximately 92% amino acid sequence homology.

Long Name

Heart And Neural Crest Derivatives Expressed 1

Entrez Gene IDs

9421 (Human); 15110 (Mouse); 59112 (Rat)

Alternate Names

BHLHA27; bHLHa27heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; eHand; Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1; HAND1; heart and neural crest derivatives expressed 1; Hxt; Thing1