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Human HDGF Antibody

Catalog #: AF1606
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1606
AF1606-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HDGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human HDGF in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HDGF
Ser2-Lys240 (Pro101Lys)
Accession # P51858
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HDGF

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HDGF

HDGF is a heparin-binding protein that was originally purified as a secreted mitogen from human HuH 7 hepatoma cells. It is the prototypic member of the HDGF family of proteins, which share a conserved N‑terminal amino acid sequence. HDGF has two putative nuclear localization signals (NLSs) and undergoes nuclear translocation.

Long Name
Hepatoma-derived Growth Factor
Entrez Gene IDs
3068 (Human)
Alternate Names
FLJ96580; HDGF; hepatoma-derived growth factor (high-mobility group protein 1-like); hepatoma-derived growth factor; High mobility group protein 1-like 2; HMG-1L2; HMG1L2DKFZp686J1764; HuHGF

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Citation for Human HDGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MiR-129-5p exerts Wnt signaling-dependent tumor-suppressive functions in hepatocellular carcinoma by directly targeting hepatoma-derived growth factor HDGF
    Authors: N Huge, T Reinkens, R Buurman, M Sandbothe, A Bergmann, H Wallaschek, B Vajen, A Stalke, M Decker, M Eilers, V Schäffer, O Dittrich-B, E Gürlevik, F Kühnel, B Schlegelbe, T Illig, B Skawran
    Cancer Cell International, 2022-05-16;22(1):192.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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