Human Hemoglobin zeta Antibody

Catalog #: MAB7708 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7708
MAB7708-SP

Detection of Human Hemoglobin zeta by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Hemoglobin zeta Antibody Summary

Species Reactivity

Human

Specificity

Detects human Hemoglobin zeta in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 778633

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Hemoglobin zeta
Met1-Arg142
Accession # P02008

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Hemoglobin zeta by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Hemoglobin zeta Monoclonal Antibody (Catalog # MAB7708) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hemoglobin zeta at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Hemoglobin zeta in K562 Human Cell Line. Hemoglobin zeta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human Hemoglobin zeta Monoclonal Antibody (Catalog # MAB7708) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hemoglobin zeta

Hemoglobin is a tetrameric heme-containing protein that is responsible for the transport of oxygen by red blood cells in the circulation. For most of fetal development and adulthood, hemoglobin consists of two alpha chains and two beta chains. Hemoglobin zeta (HBZ) is an approximately 15 kDa alpha chain-like protein that is produced during the first few weeks of embryogenesis until the onset of alpha chain expression. Its expression is prolonged in a0-thalassemia which is characterized by deficient alpha chain production. The Gower-1, Portland-1, and Portland-2 forms of hemoglobin consist of two zeta chains in association with either two epsilon, gamma, or beta chains, respectively. Human Hemoglobin zeta shares 79% amino acid sequence identity with mouse and rat Hemoglobin zeta.

Entrez Gene IDs

3050 (Human); 15126 (Mouse)

Alternate Names

HBAZ; HBZ; HBZ2; hemoglobin subunit zeta; Hemoglobin zeta chain; Hemoglobin zeta; hemoglobin, zeta; zeta-globin