Human Hepcidin Quantikine ELISA Kit

HepG2 human hepatocellular carcinoma cells were cultured in MEM supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were unstimulated or stimulated with 10 ng/mL of recombinant human IL-6, 10 ng/mL of recombinant human IL-1 beta, and 10 ng/mL of recombinant human TNF-alpha for 3 days. Aliquots of the cell culture supernates were removed, assayed for human Hepcidin, and measured 4455 pg/mL and 20,000 pg/mL, respectively.

HepG2 human hepatocellular carcinoma cells were cultured in MEM supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate under normoxic conditions or in a hypoxia workstation at 1% O2 for 3 days. Aliquots of the cell culture supernates were removed, assayed for human Hepcidin, and measured 1804 pg/mL and 3540 pg/mL, respectively.

The Quantikine Human Hepcidin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Hepcidin in cell culture supernates, serum, plasma, and urine. It contains synthetic Hepcidin and antibodies raised against synthetic Hepcidin. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring human Hepcidin.

The recovery of human Hepcidin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

To assess the linearity of the assay, samples containing high concentrations of human Hepcidin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data