Human HES-1 Antibody

Name: Human HES-1 Antibody
Brand: R&D Systems
SKU: MAB3317
Rating: 5 (1 reviews)

Catalog #: MAB3317 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3317-100
MAB3317-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HES-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HES-1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 960216

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HES-1
Asn90-Pro277
Accession # Q14469

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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HES‑1 in MCF‑7 Human Cell Line. HES-1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HES-1 Monoclonal Antibody (Catalog # MAB3317) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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HES‑1 in Human Liver Cancer Tissue. HES-1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human HES-1 Monoclonal Antibody (Catalog # MAB3317) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HES-1

HES-1 is a transcriptional repressor that is a target of notch signaling. It contains a basic helix-loop-helix (bHLH) DNA-binding domain, an Orange domain and a C‑terminal tetrapeptide WRPW motif that binds to the Groucho (Gro)/TLE/Grg family of corepressors. HES-1 can form both homo- and heterodimers with other HES family members. Dimerization is mediated through both the bHLH and the downstream Orange domain. Over the sequence used for immunization, human HES-1 shares 97% and 98% amino acid sequence identity with mouse and rat HES-1, respectively.

Long Name

Hairy And Enhancer of Split 1

Entrez Gene IDs

3280 (Human); 15205 (Mouse); 29577 (Rat)

Alternate Names

BHLHB39; bHLHb39hairy homolog (Drosophila); Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; hairy and enhancer of split 1, (Drosophila); Hairy homolog; Hairy-like protein; HES1; HES-1; hHL; HL; HRY; HRYHHL; transcription factor HES-1