Human HES-4 Antibody

Name: Human HES-4 Antibody
Brand: R&D Systems
SKU: MAB3600
Rating: 5 (1 reviews)

Catalog #: MAB3600 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3600
MAB3600-SP

Detection of Human HES‑4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HES-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HES-4 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 455237

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HES-4
Val94-Arg221
Accession # Q9HCC6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HES‑4 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human HES-4 Monoclonal Antibody (Catalog # MAB3600) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for HES-4 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HES-4

The Notch pathway maintains embryonic stem cells by up‑regulation of HES and Hey basic helix-loop-helix family members, which repress tissue specific expression. HES-4 is up‑regulated in gastric and colorectal cancers. It shares high homology with HES-1, HES-2, and HES-3.

Long Name

Hairy And Enhancer of Split 4

Entrez Gene IDs

57801 (Human)

Alternate Names

bHLH factor Hes4; BHLHB42; bHLHb42transcription factor HES-4; Class B basic helix-loop-helix protein 42; hairy and enhancer of split 4 (Drosophila); Hairy and enhancer of split 4; HES4; HES-4; hHES4