Human HGF Activator Biotinylated Antibody

Catalog #: BAF1514 Datasheet / COA / SDS

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BAF1514

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HGF Activator Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HGF Activator in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse HGF Activator is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HGF Activator
Gln36-Ser655
Accession # Q04756

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human HGF Activator (Catalog # 1514-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF Activator

Hepatocyte Growth Factor Activator (HGFA) is a serine endopeptidase that cleaves at the peptide bond between Arg494 and Val495 of single-chain human HGF precursor, generating the active heterodimer (1). HGFA is produced and secreted by the liver and normally circulates in the blood as an inactive zymogen (2, 3). The zymogen has a weak affinity for heparin but acquires a strong affinity for heparin upon activation that is linked to blood coagulation. This property may ensure the local action of this enzyme at the site of tissue injury (3). Human HGFA precursor (655 amino acid residues) contains several predicted domains including a signal peptide (residues 1‑30), a propeptide (residues 31‑372), and a mature and active form (residues 373‑655) that is further processed into a short chain (residues 373‑407) and a long chain (residues 408‑655). The short chain and the long chain (catalytic domain) may form a disulfide bond linked dimer. HGFA can be activated by thrombin (R&D Systems, Catalog # 1473-SE) or thermolysin (R&D Systems, Catalog # 3097-ZN) (4). The active protease can be inhibited by HGFA inhibitors (HAIs). Two HAIs, HAI-1 and HAI-2, are known in mouse and human. HAI-1 is not only an inhibitor, but also a specific acceptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (5).

References

Kitamura, N. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. Eds.) p. 1712, Academic Press, San Diego.
Miyazawa, K. et al. (1993) J. Biol. Chem. 268:10024.
Miyazawa, K. et al. (1996) J. Biol. Chem. 271:3615.
Shimomura, T. et al. (1993) J. Biol. Chem. 268:22927.
Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.