Human HIPK2 Antibody

Name: Human HIPK2 Antibody
Brand: R&D Systems
SKU: MAB9307
Rating: 5 (1 reviews)

Catalog #: MAB9307 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9307-100
MAB9307-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HIPK2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HIPK2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 493918

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HIPK2
Met765-Ser924
Accession # Q9H2X6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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HIPK2 in MCF‑7 Human Cell Line. HIPK2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HIPK2 Monoclonal Antibody (Catalog # MAB9307) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIPK2

Homeodomain-interacting protein kinase-2 (HIPK2) is a serine/threonine kinase which interacts with transcription factors, including p53, CREB1, SMAD1, SMAD2, RUNX1 and others, and can act as a corepressor or coactivator depending on the transcription factor and subcellular localization. Post-translational modifications control HIPK2 activity, including phosphorylation, small ubiquitin-like modifier modification, acetylation, and ubiquitination. HIPK2 regulation of cell proliferation makes it a key effector of proliferative diseases including cancer and kidney fibrosis. For example, HIPK2 can repress the proliferative Wnt-1/b-catenin pathway and HIF-1, promote apoptosis by activation of p53 and repression of delta Np63 alpha, MDM2 and CtBP, and its activity is enhanced by anti-cancer drugs such as cisplatin and etoposide. HIPK2 oncorepession can be blocked by cytoplasmic localization, hypoxia, gene mutation and loss of heterozygosity.

References

(Saul VV and Schmitz ML, J Mol Med (Berl). 2013 Sep;91(9):1051-8.) (Saul VV and Schmitz ML, J Mol Med (Berl). 2013 Sep;91(9):1051-8.) (D'Orazi G, et al, J Exp Clin Cancer Res. 2012 Aug 13;31:63. )

Long Name

Homeodomain Interacting Protein Kinase 2

Entrez Gene IDs

28996 (Human); 15258 (Mouse); 362342 (Rat)

Alternate Names

DKFZp686K02111; EC 2.7.11.1; FLJ23711; hHIPk2; HIPK2; homeodomain interacting protein kinase 2; homeodomain-interacting protein kinase 2; PRO0593; Stank