Human HLA-C Antibody

Catalog #: MAB10394 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10394-100
MAB10394-SP

Detection of HLA-C in Human PBMC Lymphocytes by Flow Cytometry.

1 Image

All HLA-C Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HLA-C Antibody Summary

Species Reactivity

Human

Specificity

Detects human HLA-C in direct ELISAs.

Source

Monoclonal Mouse IgG Clone # DT-9

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Purified tamarin MHC class I molecules (using anti-class I antibody BB7.7)
Accession # P10321

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of HLA-C in Human PBMC Lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Mouse Anti-Human HLA-C Monoclonal Antibody (Catalog # MAB10394, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HLA-C

HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.

Long Name

Major Histocompatibility Complex, Class I, C

Entrez Gene IDs

3107 (Human)

Alternate Names

C alpha chain; D6S204; FLJ27082; HLA class I histocompatibility antigen, Cw-1 alpha chain; HLAC; HLA-C; HLA-Cw; HLA-JY3; HLC-C; human leukocyte antigen-C alpha chain; leucocyte antigen C; leucocyte antigen Cw; leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C; major histocompatibility complex, class I, C; MHC class I antigen Cw*1; MHC class I antigen Cw*12; MHC class I antigen Cw*14; MHC class I antigen Cw*15; MHC class I antigen Cw*16; MHC class I antigen Cw*17; MHC class I antigen Cw*2; MHC class I antigen Cw*3; MHC class I antigen Cw*4; MHC class I antigen Cw*5; MHC class I antigen Cw*6; MHC class I antigen Cw*7; MHC class I antigen Cw*8; MHC class I antigen heavy chain HLA-C; PSORS1