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Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit

Catalog #: DHWAD0
27 Citations 2 Reviews Datasheet / COA / SDS
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Human HMW Adiponectin/Acrp30 ELISA Standard Curve
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Citations (27)
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Summary Sample Values Precision Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.989 ng/mL
Assay Range
3.9 - 250 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human high molecular weight Adiponectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human HMW Adiponectin in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard deviation (ng/mL)
Male serum (n=12)41031686-67511892
Female serum (n=24)72152595-11,8822321
Male EDTA plasma (n=12)39621539-69851926
Female EDTA plasma (n=24)68422524-10,9062215
Male heparin plasma (n=12)38891590-65071807
Female heparin plasma (n=24)68482445-11,9482286

Product Summary

The Quantikine Human HMW Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HMW Adiponectin in serum and plasma. It contains NS0-expressed recombinant human HMW Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human HMW Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human HMW Adiponectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 25.6 70.6 141 27.7 70.4 134
Standard Deviation 0.67 2.64 3.97 2.4 6 11.1
CV% 2.6 3.7 2.8 8.6 8.5 8.3

Linearity

To assess the linearity of the assay, samples containing high concentrations of HMW Adiponectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human HMW Adiponectin/Acrp30 ELISA Linearity

Scientific Data

N/A Human HMW Adiponectin/Acrp30 ELISA Standard Curve View Larger

Human HMW Adiponectin/Acrp30 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

Entrez Gene IDs:
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names:
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Effects of Exhaustive Exercise on Adiponectin and High-Molecular-Weight Oligomer Levels in Male Amateur Athletes
    Authors: Mallardo, M;Tommasini, E;Missaglia, S;Pecci, C;Rampinini, E;Bosio, A;Morelli, A;Daniele, A;Nigro, E;Tavian, D;
    Biomedicines
    Species: Human
    Sample Types: Serum
  2. Intermuscular adipose tissue accumulation is associated with higher tissue sodium in healthy individuals
    Authors: Ertuglu, LA;Sahinoz, M;Alsouqi, A;Deger, SM;Guide, A;Pike, M;Robinson-Cohen, C;Akwo, E;Pridmore, M;Crescenzi, R;Madhur, MS;Kirabo, A;Harrison, DG;Luft, FC;Titze, J;Ikizler, TA;Gamboa, JL;
    Physiological reports
    Species: Human
    Sample Types: Plasma
  3. Nicotinamide riboside improves muscle mitochondrial biogenesis, satellite cell differentiation, and gut microbiota in a twin study
    Authors: HAK Lapatto, M Kuusela, A Heikkinen, M Muniandy, BW van der Ko, S Gopalakris, N Pöllänen, M Sandvik, MS Schmidt, S Heinonen, S Saari, J Kuula, A Hakkaraine, J Tampio, T Saarinen, MR Taskinen, N Lundbom, PH Groop, M Tiirola, P Katajisto, M Lehtonen, C Brenner, J Kaprio, S Pekkala, M Ollikainen, KH Pietiläine, E Pirinen
    Science Advances, 2023-01-13;9(2):eadd5163.
    Species: Human
    Sample Types: Plasma
  4. Congenital adiponectin deficiency mitigates high-fat-diet-induced obesity in gonadally intact male and female, but not in ovariectomized mice
    Authors: CA Unger, AK Aladhami, MC Hope, S Pourhosein, M Nagarkatti, OP McGuinness, EA Murphy, KT Velázquez, RT Enos
    Scientific Reports, 2022-10-05;12(1):16668.
    Species: Human
    Sample Types: Plasma
  5. Birth Weight and Early Postnatal Outcomes: Association with the Cord Blood Lipidome
    Authors: C Gonzalez-R, M Santos, M Díaz, C García-Bel, C Lerin, C Barbas, L Ibáñez, D Sánchez-In
    Nutrients, 2022-09-12;14(18):.
    Species: Human
    Sample Types: Serum
  6. Adiponectin serum level is an independent and incremental predictor of all-cause mortality after transcatheter aortic valve replacement
    Authors: J Walpot, P van Herck, V Collas, L Bossaerts, CM Van de Hey, T Vandendrie, H Heidbuchel, I Rodrigus, C De Block, GR Small, J Bosmans
    Clinical cardiology, 2022-08-06;0(0):.
    Species: Human
    Sample Types: Serum
  7. Baseline serum biomarkers of inflammation, bone turnover and adipokines predict spinal radiographic progression in ankylosing spondylitis patients on TNF inhibitor therapy
    Authors: J Rademacher, M Siderius, L Gellert, FR Wink, M Verba, F Maas, LM Tietz, D Poddubnyy, A Spoorenber, S Arends
    Seminars in arthritis and rheumatism, 2022-02-02;53(0):151974.
    Species: Human
    Sample Types: Serum
  8. T-Cadherin and the Ratio of Its Ligands as Predictors of Carotid Atherosclerosis: A Pilot Study
    Authors: A Balatskiy, M Teterina, A Pisaryuk, I Balabanenk, A Kadrev, A Tishuk, M Balatskaya, L Samokhodsk, S Boytsov, N Kalinina, V Tkachuk
    Biomedicines, 2021-10-05;9(10):.
    Species: Human
    Sample Types: Serum
  9. Relationships between plasma apelin and adiponectin with normal weight obesity, body composition, and cardiorespiratory fitness in working adults
    Authors: MP Bellissimo, E Hsu, L Hao, K Easley, GS Martin, TR Ziegler, JA Alvarez
    Journal of clinical & translational endocrinology, 2021-06-06;24(0):100257.
    Species: Human
    Sample Types: Plasma
  10. Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
    Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
    Biomolecules, 2021-02-21;11(2):.
    Species: Human
    Sample Types: Plasma
  11. High molecular weight adiponectin levels are inversely associated with adiposity in pediatric brain tumor survivors
    Authors: R Ronsley, SR Rassekh, A Fleming, B Empringham, W Jennings, C Portwine, S Burrow, S Zelcer, DL Johnston, L Thabane, MC Samaan
    Sci Rep, 2020-10-29;10(1):18606.
    Species: Human
    Sample Types: Plasma
  12. Melinjo seed extract increases adiponectin multimerization in physiological and pathological conditions
    Authors: K Oniki, T Kawakami, A Nakashima, K Miyata, T Watanabe, H Fujikawa, R Nakashima, A Nasu, Y Eto, N Takahashi, H Nohara, MA Suico, S Kotani, Y Obata, Y Sakamoto, Y Seguchi, J Saruwatari, T Imafuku, H Watanabe, T Maruyama, H Kai, T Shuto
    Sci Rep, 2020-03-09;10(1):4313.
    Species: Human
    Sample Types: Serum
  13. A novel biomarker of cardiometabolic pathology in schizophrenia?
    Authors: EE Lee, DD Sears, J Liu, H Jin, XM Tu, LT Eyler, DV Jeste
    J Psychiatr Res, 2019-06-18;117(0):31-37.
    Species: Human
    Sample Types: Serum
  14. Air pollution and diabetes-related biomarkers in non-diabetic adults: A pathway to impaired glucose metabolism?
    Authors: S Lucht, F Hennig, S Moebus, D Führer-Sak, C Herder, KH Jöckel, B Hoffmann
    Environ Int, 2019-01-17;124(0):370-392.
    Species: Human
    Sample Types:
  15. Analysis of changes on adiponectin levels and abdominal obesity after smoking cessation
    Authors: M Komiyama, H Wada, H Yamakage, N Satoh-Asah, Y Sunagawa, T Morimoto, Y Ozaki, A Shimatsu, Y Takahashi, K Hasegawa
    PLoS ONE, 2018-08-07;13(8):e0201244.
    Species: Human
    Sample Types: Serum
  16. Effect of adiposity on tissue-specific adiponectin secretion
    Authors: J Reneau, M Goldblatt, J Gould, T Kindel, A Kastenmeie, R Higgins, LR Rengel, K Schoyer, R James, B Obi, A Moosreiner, K Nicholson, D Sahoo, S Kidambi
    PLoS ONE, 2018-06-20;13(6):e0198889.
    Species: Human
    Sample Types: Serum
  17. Serum levels of leptin and high molecular weight adiponectin are inversely associated with radiographic spinal progression in patients with ankylosing spondylitis: results from the ENRADAS trial
    Authors: A Hartl, J Sieper, U Syrbe, J Listing, KG Hermann, M Rudwaleit, D Poddubnyy
    Arthritis Res. Ther., 2017-06-15;19(1):140.
    Species: Human
    Sample Types: Serum
  18. Alterations in 3-Hydroxyisobutyrate and FGF21 Metabolism are Associated with Protein Ingestion-Induced Insulin Resistance
    Authors: LLS Harris, GI Smith, BW Patterson, RS Ramaswamy, A Okunade, SC Kelly, LC Porter, S Klein, J Yoshino, B Mittendorf
    Diabetes, 2017-05-04;0(0):.
    Species: Human
    Sample Types: Plasma
  19. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
    Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
    J Immunol Res, 2017-03-23;2017(0):6468097.
    Species: Human
    Sample Types: Plasma
  20. Effects of cold sensitivity in the extremities on circulating adiponectin levels and metabolic syndrome in women
    Authors: AY Park, S Cha
    BMC Complement Altern Med, 2017-03-09;17(1):150.
    Species: Human
    Sample Types: Serum
  21. Adiponectin, Insulin Sensitivity, ?-Cell Function, and Racial/Ethnic Disparity in Treatment Failure Rates in TODAY
    Diabetes Care, 2016-11-01;0(0):.
    Species: Human
    Sample Types: Plasma
  22. Azelnidipine, but not amlodipine, reduces urinary albumin excretion and carotid atherosclerosis in subjects with type 2 diabetes: blood pressure control with olmesartan and azelnidipine in Type 2 diabetes (BOAT2 study).
    Authors: Tawaramoto K, Kaneto H, Hashiramoto M, Kawasaki F, Tatsumi F, Shimoda M, Kamei S, Matsuki M, Mune T, Kaku K
    Diabetol Metab Syndr, 2015-09-17;7(0):80.
    Species: Human
    Sample Types: Plasma
  23. Cord blood adipokines, neonatal anthropometrics and postnatal growth in offspring of Hispanic and Native American women with diabetes mellitus.
    Authors: Teague A, Fields D, Aston C, Short K, Lyons T, Chernausek S
    Reprod Biol Endocrinol, 2015-06-26;13(0):68.
    Species: Human
    Sample Types: Serum
  24. Gender-Specific Associations between Circulating T-Cadherin and High Molecular Weight-Adiponectin in Patients with Stable Coronary Artery Disease.
    Authors: Schoenenberger A, Pfaff D, Dasen B, Frismantiene A, Erne P, Resink T, Philippova M
    PLoS ONE, 2015-06-17;10(6):e0131140.
    Species: Human
    Sample Types: Plasma
  25. Adding glimepiride to current insulin therapy increases high-molecular weight adiponectin levels to improve glycemic control in poorly controlled type 2 diabetes.
    Authors: Li C, Zhang J, Yu D, Zhang Q
    Diabetol Metab Syndr, 2014-03-20;6(1):41.
    Species: Human
    Sample Types: Plasma
  26. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
    Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
    PLoS ONE, 2013-12-10;8(12):e83042.
    Species: Human
    Sample Types: Plasma
  27. A prospective study of circulating adipokine levels and risk of multiple myeloma.
    Authors: Hofmann J, Liao L, Pollak M, Wang Y, Pfeiffer R, Baris D, Andreotti G, Lan Q, Landgren O, Rothman N, Purdue M
    Blood, 2012-09-24;120(22):4418-20.
    Species: Human
    Sample Types: Plasma

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Reviews for Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit

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Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
By Anonymous on 11/13/2018
Sample Tested: Adipose tissue
Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit DHWAD0
Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
By Anonymous on 08/03/2017
Sample Tested: Serum

sample dilution used-1:100

Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit DHWAD0