Human HNF-4 alpha/NR2A1 Antibody

Catalog #: PP-H6939-00
10 Citations Datasheet / COA / SDS

Isoforms 7-9

Catalog #	Availability	Size / Price	Qty
PP-H6939-00

Product Details

Citations (10)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HNF-4 alpha/NR2A1 Antibody Summary

Species Reactivity

Human

Source

Monoclonal Mouse IgG1 Clone # H6939

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Please refer to the PDF datasheet for specifications.

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human large intestine

Immunoprecipitation

Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HNF-4 alpha/NR2A1

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Entrez Gene IDs

3172 (Human)

Alternate Names

FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; hepatocyte nuclear factor 4-alpha; HNF4 alpha; HNF-4 alpha; HNF4A; HNF4a7; HNF-4-alpha; HNF4alpha10/11/12; HNF4HNF4a8; MODY; MODY1; NR2A1; NR2A1HNF4a9; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF; TCF14; TCF-14; TCF14HNF4alpha; Transcription factor 14; Transcription factor HNF-4; transcription factor-14

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human HNF-4 alpha/NR2A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

Diet high in linoleic acid dysregulates the intestinal endocannabinoid system and increases susceptibility to colitis in Mice
Authors: Poonamjot Deol, Paul Ruegger, Geoffrey D. Logan, Ali Shawki, Jiang Li, Jonathan D. Mitchell et al.
Gut Microbes
Analysis of the HNF4A isoform-regulated transcriptome identifies CCL15 as a downstream target in gastric carcinogenesis
Authors: Zhen Ni, Wenquan Lu, Qi Li, Chuan Han, Ting Yuan, Nina Sun et al.
Cancer Biology and Medicine
CREB stimulates GPX4 transcription to inhibit ferroptosis in lung adenocarcinoma
Authors: Z Wang, X Zhang, X Tian, Y Yang, L Ma, J Wang, Y Yu
Oncology reports, 2021-04-13;45(6):.
Species: Human
Sample Types: Cell Lysates
Applications: ChIP
Ferroptosis is governed by differential regulation of transcription in liver cancer
Authors: X Zhang, L Du, Y Qiao, X Zhang, W Zheng, Q Wu, Y Chen, G Zhu, Y Liu, Z Bian, S Guo, Y Yang, L Ma, Y Yu, Q Pan, F Sun, J Wang
Redox Biol, 2019-05-10;24(0):101211.
Species: Human
Sample Types: Cell Lysates
Applications: ChIP, Immunoprecipitation, Proximity Ligation Assay
Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity
Authors: PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal
Dev. Cell, 2018-03-26;44(6):679-693.e5.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
TET-Catalyzed 5-Hydroxymethylation Precedes HNF4A Promoter Choice during Differentiation of Bipotent Liver Progenitors
Authors: PB Ancey, S Ecsedi, MP Lambert, FR Talukdar, MP Cros, D Glaise, DM Narvaez, V Chauvet, Z Herceg, A Corlu, H Hernandez-
Stem Cell Reports, 2017-06-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Opposing roles of nuclear receptor HNF4 alpha isoforms in colitis and colitis-associated colon cancer
Authors: Karthikeyani Chellappa, Poonamjot Deol, Jane R Evans, Linh M Vuong, Gang Chen, Nadege Briançon et al.
eLife
Differential Effects of Hepatocyte Nuclear Factor 4 alpha Isoforms on Tumor Growth and T-Cell Factor 4/AP-1 Interactions in Human Colorectal Cancer Cells
Authors: Linh M. Vuong, Karthikeyani Chellappa, Joseph M. Dhahbi, Jonathan R. Deans, Bin Fang, Eugene Bolotin et al.
Molecular and Cellular Biology
Mutant IDH inhibits HNF-4alpha to block hepatocyte differentiation and promote biliary cancer.
Authors: Saha S, Parachoniak C, Ghanta K, Fitamant J, Ross K, Najem M, Gurumurthy S, Akbay E, Sia D, Cornella H, Miltiadous O, Walesky C, Deshpande V, Zhu A, Hezel A, Yen K, Straley K, Travins J, Popovici-Muller J, Gliser C, Ferrone C, Apte U, Llovet J, Wong K, Ramaswamy S, Bardeesy N
Nature, 2014-07-02;513(7516):110-4.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Novel P2 promoter-derived HNF4alpha isoforms with different N-terminus generated by alternate exon insertion.
Authors: Huang J, Levitsky LL, Rhoads DB
Exp. Cell Res., 2009-04-15;315(7):1200-11.
Species: Human
Sample Types: Cell Lysates
Applications: Mobility Shift Assay