Human HNF-6/ONECUT1 Antibody Summary
Gln139-Gly283
Accession # Q9UBC0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human HNF‑6/ONECUT1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for HNF-6/ONECUT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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HNF‑6/ONECUT1 in HepG2 Human Cell Line. HNF-6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of HNF‑6/ONECUT1 in HepG2 (positive) and HDLM-2 (negative) cells. HNF‑6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cells (positive) and absent in HDLM‑2 human Hodgkin’s lymphoma cells (negative) using Sheep Anti-Human HNF‑6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HNF-6/ONECUT1
HNF-6 (also ONECUT1; one "cut" domain family member 1; OC-1) is a monomeric 53-56 kDa member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic hepatic and pancreatic islet anlage, plus adult hepatocytes and intestinal epithelial cells. HNF-6 is a transcriptional activator that is known to interact with Foxa2. Genes regulated by HNF-6 include Glut2, alpha 1AT, alpha -fetoprotein and HNF-3 beta. Human HNF-6 is 465 amino acids (aa) in length. It contains two DNA-binding regions, one classified as a CUT domain (aa 283-369) and another as a homeobox domain (aa 385-444). HNF-6 is both acetylated and phosphorylated, contributing to protein stability and transactivation activity. Over aa 139-283, human HNF 6 shares 99% aa identity with mouse HNF-6. Rat HNF-6 shows a 26 aa insertion after Ala368. Given an overall aa identity of 99% between rat and human, the possibility exists that this insertion may also exist in human.
Product Datasheets
Citations for Human HNF-6/ONECUT1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord
Authors: Audrey Harris, Gauhar Masgutova, Amandine Collin, Mathilde Toch, Maria Hidalgo-Figueroa, Benvenuto Jacob et al.
Frontiers in Cellular Neuroscience
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Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits
Authors: M Toch, A Harris, O Schakman, E Kondratska, JL Boulland, N Dauguet, S Debrulle, C Baudouin, M Hidalgo-Fi, A Gow, JC Glover, F Tissir, F Clotman
Sci Rep, 2020-01-22;10(1):996.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development
Authors: KU Kabayiza, G Masgutova, A Harris, V Rucchin, B Jacob, F Clotman
Front Mol Neurosci, 2017-05-26;10(0):157.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Vsx1 Transiently Defines an Early Intermediate V2 Interneuron Precursor Compartment in the Mouse Developing Spinal Cord
Authors: Cédric Francius, María Hidalgo-Figueroa, Stéphanie Debrulle, Barbara Pelosi, Vincent Rucchin, Kara Ronellenfitch et al.
Frontiers in Molecular Neuroscience
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Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord
Authors: Gauhar Masgutova, Audrey Harris, Benvenuto Jacob, Lynn M. Corcoran, Frédéric Clotman
Frontiers in Molecular Neuroscience
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