Home / Holo-Transferrin / Human Holo-Transferrin Protein, CF

Human Holo-Transferrin Protein, CF

Catalog #: 2914-HT
51 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2914-HT-001G
2914-HT-100MG
Product Details
Citations (51)
FAQs
Supplemental Products
Reviews (4)
Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Human Holo-Transferrin Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. Taub, M. et al. (1979) PNAS 76:3338. The ED50 for this effect is 0.075-0.375 μg/mL.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Human plasma-derived Holo-Transferrin protein

The human plasma used for the isolation of this product were certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.

SDS-PAGE
76-81 kDa, reducing conditions

Product Datasheets

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2914-HT

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2914-HT

Formulation Lyophilized from a 0.2 μm filtered solution in sterile water.
Reconstitution Reconstitute at 20 mg/mL in sterile, deionized water.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Holo-Transferrin

Human Transferrin (Tf) is a single chain, 80 kDa member of the anion-binding superfamily of proteins (1 - 5). It is a bilobed molecule that is the product of an ancient gene duplication event (1, 6). Transferrin is synthesized as a 698 amino acid (aa) precursor that is divided into a 19 aa signal sequence plus a 679 aa mature segment that contains 19 intrachain disulfide bonds. The crystal structure of Tf reveals a protein with two flanking 340 aa globular domains. Each are composed of a beta -sheet surrounded by series of alpha -helices (1, 7). The N- and C-terminal flanking regions (or domains) will bind ferric iron through the interaction of an obligate anion (usually bicarbonate) and four amino acids (His, Asp, and two Tyr) (7, 8). Apotransferrin (or iron‑free) will initially bind one atom of iron at the C-terminus, and this is followed by subsequent iron binding by the N‑terminus to form holotransferrin (diferric Tf) (8, 9). Through its C-terminal iron‑binding domain, holotransferrin will interact with the type I Tf receptor (TfR) on the surface of cells where it is internalized into acidified endosomes. Iron dissociates from the Tf molecule within these endosomes, and is transported into the cytosol as ferrous iron. At physiological pH, iron‑free apotransferrin is not bound by TfR. But at acidic pH, such as exists in the endosome, apotransferrin has considerable affinity for TfR. Thus, it remains bound to TfR and is recycled back to the cell surface where a neutral pH environment dissociates ligand from receptor. Each Tf molecule recycles 100 - 150 times during its lifetime (8 - 11). In addition to TfR, transferrin is reported to bind to cubulin, IGFBP3, microbial iron‑binding proteins and liver-specific TfR2 (7, 12, 13, 14). Transferrin is variably glycosylated and the degree of sialylation is suggestive of certain clinical conditions (15). Finally, Tf is highly allelic and the gene codominant, with many single aa changes noted. Three general forms are known, based on standard electrophoretic mobility. Fast Tf is known as transferrin B, slow transferrin is transferrin D, and the middle migrating transferrin is type/variant C, thre most common (16, 17). Mature human TF is 73% aa identical to both mouse and rat Tf, and 68% and 71% aa identical to bovine and equine Tf, respectively.

References
  1. Brus, C.M. et al. (2001) Nat. Struct. Biol. 4:919.
  2. Schaeffer, E. et al. (1987) Gene 56:109.
  3. MacGillivray, R.T.A. et al. (1983) J. Biol. Chem. 258:3543.
  4. Yang, F. et al. (1984) Proc. Natl. Acad. Sci. USA 81:2752.
  5. Uzan, G. et al. (1984) Biochem. Biophys. Res. Commun. 119:273.
  6. Zak, O. et al. (2002) Biochemistry 41:7416.
  7. Gomme, P.T. and K. B. McCann (2005) Drug Discov. Today 10:267.
  8. Liu, R. et al. (2003) Biochemistry 42:12447.
  9. Pakdaman, R. et al. (1999) J. Mol. Biol. 293:1273.
  10. Hemadi, M. et al. (2004) Biochemistry 43:1736.
  11. Aisen, P. et al. (2001) Int. J. Biochem. Cell Biol. 33:940.
  12. Kozyraki, R. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12941.
  13. Boulton, I.C. et al. (1998) Biochem. J. 334:269.
  14. Robb, A. and M. Wessling-Resnick (2004) Blood 104:4294.
  15. Landberg, E. et al. (1995) Biochem. Biophys. Res. Commun. 210:267.
  16. Gorg, A. et al. (1983) Hum. Genet. 64:222.
  17. Bean, P. and J.B. Peter (1994) Clin. Chem. 40:2078.
Entrez Gene IDs
7018 (Human)
Alternate Names
HoloTransferrin; Holo-Transferrin

Citations for Human Holo-Transferrin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

51 Citations: Showing 1 - 10
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  1. Establishment of a transgene-free iPS cell line (SDCHi008-A) from a young patient bearing a KCNQ2 mutation and suffering from Epilepsy
    Authors: Zhang, H;Zhang, Q;Chen, X;Wang, M;Li, L;Yu, C;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Establishment of a transgene-free iPS cell line (SDCHi007-A) from a young patient bearing a ATP1A2 mutation and suffering from Epilepsy
    Authors: Zhang, H;Zhang, T;Wang, Y;Shi, J;Meng, Y;Zhang, Q;Guo, Q;Duan, C;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Emergence of large-scale cell death through ferroptotic trigger waves
    Authors: Co, HKC;Wu, CC;Lee, YC;Chen, SH;
    Nature
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. INA03, a potent transferrin-competitive antibody-drug conjugate against CD71, for a safer acute leukemia treatment
    Authors: Bratti, M;Stubbs, E;Kolodych, S;Souchet, H;Kelly, L;Merlin, J;Marchal, M;Castellano, R;Josselin, E;Pasquer, H;Benajiba, L;Puissant, A;Koniev, O;Collette, Y;Belanger, C;Hermine, O;Monteiro, RC;Launay, P;
    Molecular cancer therapeutics
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Establishment of a non-integrated iPSC line (SDQLCHi043-A) from a male infant with propionic acidemia carrying compound heterozygote mutations in PCCB gene
    Authors: Li, Z;Liu, C;Xin, H;Lv, Y;Gao, M;Ma, J;Liu, N;Gai, Z;Liu, Y;
    Stem cell research
    Species: Human
    Sample Types: BALF
    Applications: Bioassay
  6. Generation and characterization of PBMCs-derived human induced pluripotent stem cell (iPSC) line SDQLCHi050-A from a healthy donor
    Authors: Yang, X;Fan, Y;Li, Y;Zhang, H;Wang, B;Guan, J;Gao, J;Ma, X;Liu, Y;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. An induced pluripotent stem cell line (SDQLCHi062-A) from a patient carrying a mutation in the PAX2 gene
    Authors: Yang, X;Zhang, H;Gao, M;Lv, Y;Song, W;Duan, C;Liu, Y;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Establishment of human induced pluripotent stem cell line (SDQLCHi060-A) from a patient with AUTS2 syndrome carrying a heterozygous mutation in AUTS2 gene
    Authors: Gao, C;Liu, Y;Liu, N;Li, Z;Yang, X;Yang, Y;Xi, Y;Tian, J;Gai, Z;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Generation of a transgene-free iPS cell line (SDQLCHi053-A) from a young girl carrying a heterozygous mutation (c.427C > T) in SYNGAP1 gene
    Authors: Wang, Y;Yang, X;Zhang, H;Liu, N;Liu, Y;Gai, Z;Liu, Y;Lv, Y;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Generation and characterization of PBMCs-derived human induced pluripotent stem cell (iPSC) line SDQLCHi051-A from an autism spectrum disorder patient with compound CHD8 gene mutations
    Authors: Fan, Y;Li, Y;Yang, X;Zhang, H;Wang, B;Guan, J;Gao, J;Ma, X;Liu, Y;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Direct correction of haemoglobin E beta-thalassaemia using base editors
    Authors: M Badat, A Ejaz, P Hua, S Rice, W Zhang, LD Hentges, CA Fisher, N Denny, R Schwessing, N Yasara, NBA Roy, F Issa, A Roy, P Telfer, J Hughes, S Mettananda, DR Higgs, JOJ Davies
    Nature Communications, 2023-04-19;14(1):2238.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Generation of an induced pluripotent stem cell line SDPHi002-A from a patient with lung cancer
    Authors: D Ai, X Yang, X Li, N Liu, X Xie, Y Liu, W Song
    Stem Cell Research, 2023-04-15;69(0):103096.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Establishment of an induced pluripotent stem cell line SDQLCHi049-A from a healthy male neonate
    Authors: Y Xi, H Zhang, B Wang, W Song, Y Liu
    Stem Cell Research, 2023-04-15;69(0):103097.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Evaluation of 2D and 3D Erythroid Differentiation Protocols Using Sickle Cell Disease and Healthy Donor Induced Pluripotent Stem Cells
    Authors: Martins, GLS;Nonaka, CKV;Rossi, EA;de Lima, AVR;Adanho, CSA;Oliveira, MS;Yahouedehou, SCMA;de Souza, CLEM;Gon�alves, MS;Paredes, BD;Souza, BSF;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Sustained systemic inflammation increases autophagy and induces EMT/fibrotic changes in mouse liver cells: Protection by melatonin
    Authors: O Chowdhury, S Ghosh, A Das, H Liu, P Shang, NA Stepicheva, S Hose, D Sinha, S Chattopadh
    Cellular Signalling, 2022-11-12;101(0):110521.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  16. Generation of an induced pluripotent stem cell line (SDQLCHi044-A) from a patient with autosomal dominant mental retardation type 5 harboring heterozygous mutation in SYNGAP1 gene
    Authors: Y Wang, Y Lv, X Yang, Y Li, Z Li, Z Gao, Z Gai, Y Liu
    Stem Cell Research, 2022-09-17;64(0):102922.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. The chromatin remodeller ATRX facilitates diverse nuclear processes, in a stochastic manner, in both heterochromatin and euchromatin
    Authors: J Truch, DJ Downes, C Scott, ER Gür, JM Telenius, E Repapi, R Schwessing, M Gosden, JM Brown, S Taylor, PL Cheong, JR Hughes, DR Higgs, RJ Gibbons
    Nature Communications, 2022-06-17;13(1):3485.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Reticulocyte Maturation and Variant Red Blood Cells
    Authors: CJ Stevens-He, JF Flatt, S Kupzig, LJ Bruce
    Frontiers in Physiology, 2022-03-07;13(0):834463.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. MTAP-related increased erythroblast proliferation as a mechanism of polycythaemia vera
    Authors: C Tipgomut, A Khuhapinan, MC Wilson, S Poldee, KJ Heesom, C Metheetrai, O Sripichai, C Mitrpant, J Frayne, K Trakarnsan
    Scientific Reports, 2021-11-18;11(1):22483.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Nonclinical safety evaluation of pabinafusp alfa, an anti-human transferrin receptor antibody and iduronate-2-sulfatase fusion protein, for the treatment of neuronopathic mucopolysaccharidosis type II
    Authors: R Yamamoto, E Yoden, N Tanaka, M Kinoshita, A Imakiire, T Hirato, K Minami
    Molecular genetics and metabolism reports, 2021-04-18;27(0):100758.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  21. Generation of an induced pluripotent stem cell line SDQLCHi026-A from a hereditary tyrosinemia type I patient carrying compound heterozygote mutations in FAH gene
    Authors: H Zhang, C Liu, Y Ma, L Lin, Y Lv, M Gao, Z Gai, Y Liu
    Stem Cell Research, 2021-04-09;53(0):102331.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene
    Authors: Z Li, X Zhang, X Li, Y Yang, H Xin, X Yang, N Liu, Z Gai, Y Liu
    Stem Cell Research, 2021-03-26;53(0):102313.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  23. Type IV pilus retraction enables sustained bacteremia and plays a key role in the outcome of meningococcal sepsis in a humanized mouse model
    Authors: JP Barnier, D Euphrasie, O Join-Lambe, M Audry, S Schonherr-, T Schmitt, S Bourdoulou, M Coureuil, X Nassif, M El Behi
    PloS Pathogens, 2021-02-16;17(2):e1009299.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  24. Reprogramming of human peripheral blood mononuclear cell (PBMC) from a patient suffering from hearing loss into iPSC line (SDQLCHi035-A) maintaining compound heterozygous variations in GJB2 gene
    Authors: X Yang, N Liu, H Mu, Y Lv, H Zhang, Y Li, J Guan, Z Gai, Y Liu
    Stem Cell Research, 2021-01-21;51(0):102188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Establishment of a human induced pluripotent stem cell line (SDQLCHi037-A) from a patient with Alagille syndrome carrying heterozygous mutation in JAG1 gene
    Authors: B Wang, L Yang, Y Li, M Gao, H Zhang, X Yang, J Guan, Y Liu, Z Gai
    Stem Cell Research, 2021-01-08;51(0):102162.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Generation and characterization of an induced pluripotent stem cell line SDQLCHi018-A from a congenital myasthenic syndrome patient carrying compound heterozygote mutations in RAPSN gene
    Authors: H Zhang, H Zhang, Y Ma, Y Lv, Z Gai, Y Liu
    Stem Cell Research, 2021-01-08;51(0):102160.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. PBMC-derived integration-free iPSCs line SDQLCHi039-A from a patient with X-linked agammaglobulinemia carrying a novel 9-bp in-frame deletion in BTK gene
    Authors: N Liu, X Yang, S Wang, R Dong, Y Li, Y Lv, Y Liu, Z Gai
    Stem Cell Research, 2021-01-08;51(0):102165.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. An induced pluripotent stem cell line (SDQLCHi033-A) derived from a patient with maple syrup urine disease type Ib carrying a homozygous mutation in BCKDHB gene
    Authors: B Wang, C Liu, H Zhang, Z Gai, Y Liu
    Stem Cell Research, 2020-12-28;50(0):102146.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Establishment of a non-integrate iPS cell line (SDQLCHi023-A) from a patient with Xq25 microduplication syndrome carrying a 1.3�Mb hemizygote duplication at chrXq25
    Authors: X Yang, C Duan, H Zhang, Y Li, J Guan, D Wang, Y Lv, Z Gai, Y Liu
    Stem Cell Research, 2020-12-28;51(0):102147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Stage Specific Expression Pattern of Alpha-Hemoglobin-Stabilizing-Protein (AHSP) Portrayed in Erythroblast Chronology
    Authors: J Walczak, MD Camargo Jo, K Muthumalai
    Methods Protoc, 2020-06-30;3(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  31. Cystatin B is essential for proliferation and interneuron migration in individuals with EPM1 epilepsy
    Authors: F Di Matteo, F Pipicelli, C Kyrousi, I Tovecci, E Penna, M Crispino, A Chambery, R Russo, AC Ayo-Martin, M Giordano, A Hoffmann, E Ciusani, L Canafoglia, M Götz, R Di Giaimo, S Cappello
    EMBO Mol Med, 2020-05-07;0(0):e11419.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Using inducible lentiviral vectors to generate induced pluripotent stem cell line ZOCi001-A from peripheral blood cells of a patient with CRB1-/- retinitis pigmentosa
    Authors: X Tang, X Liu, Z Chen, L Luo, X Liu, J Deng, DW Li, Y Liu
    Stem Cell Res, 2020-04-30;45(0):101817.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  33. Expression of South East Asian Ovalocytic Band 3 Disrupts Erythroblast Cytokinesis and Reticulocyte Maturation
    Authors: JF Flatt, CJ Stevens-He, NM Cogan, DJ Eggleston, NM Haines, KJ Heesom, V Picard, C Thomas, LJ Bruce
    Front Physiol, 2020-04-28;11(0):357.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  34. An integration-free iPSC line SDQLCHi025-A from a girl with multiminicore disease carrying compound heterozygote mutations in RYR1 gene
    Authors: H Zhang, Y Ma, Y Lv, Y Wan, Q Zhao, Z Gai, Y Liu
    Stem Cell Res, 2020-03-20;45(0):101775.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Generation of patient-specific pluripotent induced stem cell line SDUBMSI002-A from a patient with X-linked mental retardation syndrome
    Authors: X Liu, X Yang, Y Li, X Wang, J Ma, W Jiang, Y Liu, W Sun, Y Gong
    Stem Cell Res, 2020-02-05;43(0):101724.
    Species: Human
    Sample Types: Whole Cells
  36. An integration-free iPSC line (SDQLCHi017-A) derived from a patient with nemaline myopathy-2 disease carrying compound heterozygote mutations in NEB gene
    Authors: Y Ma, H Zhang, X Li, X Yang, Y Li, J Guan, Y Lv, Z Gai, Y Liu
    Stem Cell Res, 2020-02-04;43(0):101729.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  37. Generation of a human induced pluripotent stem cell line (SDUBMSi001-A) from a hereditary spastic paraplegia patient carrying kif1a c.773C>T missense mutation
    Authors: W Xiaojing, M Yanyan, D Ruonan, L Xiaolin, Z Haiyan, M Jian, L Yi, S Wenjie, L Qiji
    Stem Cell Res, 2020-02-04;43(0):101727.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  38. Generation of an induced pluripotent stem cell line (SDQLCHi009-A) from a patient with 47,XXY and ornithine transcarbamylase deficiency carrying a hemizygote mutation in OTC
    Authors: X Yang, B Yan, H Zhang, Y Ma, Q Zhou, Y Li, J Guan, D Wang, Y Liu, Z Gai
    Stem Cell Res, 2020-01-17;43(0):101704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Generation of a Human iPSC line (SDQLCHi021-A) from a patient with methylmalonic acidemia cblC type carrying compound heterozygous mutations in MMAHC gene
    Authors: J Guan, Z Li, H Zhang, X Yang, Y Ma, Y Li, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2020-01-16;43(0):101709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  40. Establishment of a human iPSC line (SDQLCHi010-A) from a patient with optic nerve malformation carrying a heterozygous mutation in PAX6 gene
    Authors: H Zhang, Y Ma, S Yu, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2019-10-21;41(0):101611.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  41. An integration-free iPSC line (SDQLCHi013-A) derived from a patient with maple syrup urine disease carrying compound heterozygote mutations in BCKDHA gene
    Authors: H Zhang, Y Ma, X Li, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2019-10-12;41(0):101585.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  42. An induced pluripotent stem cell line (SDQLCHi006-A) derived from a patient with maple syrup urine disease type Ib carrying compound heterozygous mutations of p.R168C and p.T322I in BCKDHB gene
    Authors: Y Li, H Zhang, B Yan, Y Ma, X Yang, J Guan, Y Lv, M Gao, J Ma, Z Gai, Y Liu
    Stem Cell Res, 2019-09-14;40(0):101579.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  43. An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
    Authors: Y Ma, H Zhang, S Zhang, R Dong, X Yang, Y Li, J Guan, Z Gai, Y Liu
    Stem Cell Res, 2019-09-13;41(0):101577.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  44. Establishment of a human induced pluripotent stem cell line (SDQLCHi004-A) from a patient with nemaline myopathy-4 disease carrying heterozygous mutation in TPM2 gene
    Authors: Y Ma, H Zhang, X Yang, Y Li, J Guan, Y Lv, H Li, Y Liu, Z Gai
    Stem Cell Res, 2019-09-10;40(0):101559.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Highly Efficient and Marker-free Genome Editing of Human Pluripotent Stem Cells by CRISPR-Cas9 RNP and AAV6 Donor-Mediated Homologous Recombination
    Authors: RM Martin, K Ikeda, MK Cromer, N Uchida, T Nishimura, R Romano, AJ Tong, VT Lemgart, J Camarena, M Pavel-Dinu, C Sindhu, V Wiebking, S Vaidyanath, DP Dever, RO Bak, A Laustsen, BJ Lesch, MR Jakobsen, V Sebastiano, H Nakauchi, MH Porteus
    Cell Stem Cell, 2019-05-02;24(5):821-828.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  46. Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease
    Authors: K Denis, M Le Bris, L Le Guennec, JP Barnier, C Faure, A Gouge, H Bouzinba-S, A Jamet, D Euphrasie, B Durel, N Barois, P Pelissier, PC Morand, M Coureuil, F Lafont, O Join-Lambe, X Nassif, S Bourdoulou
    Nat Microbiol, 2019-03-25;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  47. Optimization of an erythroid culture system to reduce the cost of in vitro production of red blood cells
    Authors: S Poldee, C Metheetrai, S Nugoolsuks, J Frayne, K Trakarnsan
    MethodsX, 2018-11-30;5(0):1626-1632.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage
    Authors: BM Hopkinson, C Desler, M Kalisz, PS Vestentoft, L Juel Rasmu, HC Bisgaard
    Oxid Med Cell Longev, 2017-02-06;2017(0):5080128.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Experimental evidences of human coronary microvasculature and myocardial tissue bacterial colonization during meningococcemia
    Infect Immun, 2016-09-19;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  50. Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs After Genome Editing of the Sickle Point Mutation.
    Authors: Huang X, Wang Y, Yan W, Smith C, Ye Z, Wang J, Gao Y, Mendelsohn L, Cheng L
    Stem Cells, 2015-05-01;33(5):1470-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Systemic combinatorial peptide selection yields a non-canonical iron-mimicry mechanism for targeting tumors in a mouse model of human glioblastoma.
    Authors: Staquicini FI, Ozawa MG, Moya CA, Driessen WH, Barbu EM, Nishimori H, Soghomonyan S, Flores LG, Liang X, Paolillo V, Alauddin MM, Basilion JP, Furnari FB, Bogler O, Lang FF, Aldape KD, Fuller GN, Höök M, Gelovani JG, Sidman RL, Cavenee WK, Pasqualini R, Arap W
    J. Clin. Invest., 2010-12-22;121(1):161-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What are the differences between Human Holo-Transferrin Protein, CF (Catalog # 2914-HT) and Human Apo-Transferrin Protein, CF (Catalog # 3188-AT)?

    • Human Transferrin has two different iron binding domains, one at the N-terminus and one at the C-terminus. Apo-transferrin (3188-AT) is not bound to any iron atoms and Holo-Transferrin (2914-HT) has an iron atom bound at both the N- and C-terminal domains.

  2. When I opened the vial of Human Holo-Transferrin Protein, CF (catalog # 2914-HT), the white powder turned red in color - is that normal?

    • The holo-transferrin proteinis saturated with iron and the appearance of pink to dark orange/red color is consistent with our manufacturing observations.

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Reviews for Human Holo-Transferrin Protein, CF

Average Rating: 4.5 (Based on 4 Reviews)

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Human Holo-Transferrin Protein, CF
By Anonymous on 06/03/2022
Application: Media additive for protein or antibody production
Reason for Rating: Human Holo-Transferrin Protein was used to produce protein in the culture using HEK293.
Media additive for protein or antibody production Human Holo-Transferrin Protein, CF 2914-HT
Human Holo-Transferrin Protein, CF
By Anonymous on 03/01/2022
Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: Human Holo-Transferrin Protein was used to promote the formation of the cyst of the stem cells.
Stem/Immune cell maintenance or differentiation Human Holo-Transferrin Protein, CF 2914-HT
Human Holo-Transferrin Protein, CF
By Anonymous on 12/15/2016
Application: Stem/Immune cell maintenance or differentiation

We use human Transferrin to supplement media for murine embryonic stem cells (ESC) differentiation in embryoid bodies (EBs) and further to hemogenic endothelium from which hematopoietic stem and progenitor cells emerge.

Human Holo-Transferrin Protein, CF
By Anonymous on 01/28/2016
Human Holo-Transferrin Protein, CF 2914-HT