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Human HSP47 Antibody

Catalog #: MAB91661
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB91661-SP
MAB91661-100
Detection of Human HSP47 by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HSP47 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HSP47 in direct ELISAs and Western blots.  In Western blots, no cross-reactivity with mouse HSP47 is observed.
Source
Monoclonal Mouse IgG2B Clone # 950811
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human HSP47
Ala19-Asp412
Accession # P50454
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HSP47 antibody by Western Blot. View Larger

Detection of Human HSP47 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP47 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HSP47 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

HSP47 in BG01V Human Embryonic Stem Cells. HSP47 was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry HSP47 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

HSP47 in Human Breast Cancer Tissue. HSP47 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP47

Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.  In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X.  Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.  Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.

References
  1. Christiansen HE, et al, (2010) Am. J. Hum. Genet. 86:3892.
  2. Tasab M, et al, (2000) EMBO J. 19:22043.
  3. Kwon YJ, et al, (2009) Oncol Res. 18:1414.
  4. Zhu J, et al, (2015) Cancer Res. 75:15805.
  5. Nese N, et al, (2010) Anal Quant Cytol Histol. 32:90
Long Name
Heat Shock Protein 47
Entrez Gene IDs
871 (Human); 12406 (Mouse); 29345 (Rat)
Alternate Names
(collagen binding protein 1); Arsenic-transactivated protein 3; AsTP3; BERF-1; CBP1; CBP2; CBP2RA-A47; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; colligen; Colligin; colligin-1; colligin-2; gp46; HSP47; HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); member 1, (collagen binding protein 1); member 2; member 2, (collagen-binding protein 2); OI10; PPROM; proliferation-inducing gene 14; RA-A47; rheumatoid arthritis antigen A-47,47 kDa heat shock protein; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1; SERPINH1; SERPINH2colligin

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Citation for Human HSP47 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A comprehensive library of human transcription factors for cell fate engineering
    Authors: AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church
    Nature Biotechnology, 2020-11-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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