Human HSP90 alpha Antibody

Catalog #: AF7247
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7247
AF7247-SP

Detection of Human HSP90 alpha by Western Blot.

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All HSP90 alpha Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HSP90 alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human HSP90 alpha in Western blots. In Western blots no cross-reactivity with recombinant human HSP90 beta is observed.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Human HSP90 alpha isoform 1 synthetic peptide
Asp702-Met716
Accession # P07900

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Knockout Validated

HSP90 alpha is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in HSP90 alpha knockout HEK293T cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HSP90 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human HSP90a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7247) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant human HSP90a and recombinant human HSP90 beta (5 ng/lane) were included. A specific band was detected for HSP90a at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human HSP90a Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human HSP90 alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HSP90a knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human HSP90a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7247) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP90a at approximately 96 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP90 alpha

The heat shock protein-90 kDa (HSP90) is a composite name for a large group of genes whose molecular weights average 90 kDa. HSP90 functions primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP90 is ubiquitously expressed, highly conserved and accounts for 1‑2% of the total cellular protein. Recently introduced, standardized nomenclature has divided the 17 identified HSP90 genes into three related and one unrelated classes, HSP90AA1, HSP90AB, HSP90BB, and TRAP, respectively. Six of these genes were functional while the remaining 11 are considered putative pseudogenes. Eukaryotic cells have two principal forms of HSP90. The AF7247 antibody described here is specific to HSP90 the inducible form, HSP90AA1, also known as HSP90 alpha, HSP90A, HSPC1, HSPCA and HSP86. The other form is a constitutively expressed HSP90AB1 that is a 724 amino acid protein that is also known as HSP90-beta, HSP90B, HSPCB, HSPC2, and HSP89-beta. HSP90AB1-1 and HSP90AA share 90% identity. In addition to its role as a molecular chaperone and stress response protein, HSP90 is a central component in a number of basic cellular processes including hormone signaling and cell cycle control.

Long Name

Heat Shock Protein 90 alpha

Entrez Gene IDs

3320 (Human); 15519 (Mouse); 299331 (Rat)

Alternate Names

FLJ31884; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein HSP 90-alpha; HSP86; Hsp89; HSP89A; HSP90 alpha; Hsp90; HSP90A; HSP90AA1; HSP90AHSP86; HSP90N; HSPC1; HSPC1HSP 86; HSPCA; HSPCAHSP89A; HSPCAL1; HSPCAL4; HSPN; LAP2; Renal carcinoma antigen NY-REN-38

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human HSP90 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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